pcr克隆 meaning in English
pcr cloning
Examples
- To our surprise , one of the products contained the wrky domain , and the other not
根据pcr克隆结果，表明osdd2基因在这个文库中可能存在两种剪接方式。 - In the experiment , the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked . it was expressed in e . coli and its protein was determined . after having been properly modified , the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing , which is the prerequisite work for genetic transformation
本实验从抗除草剂转基因bobwhite小麦中，利用pcr克隆的方法扩增出bar基因全长，并在原核表达系统中表达，鉴定表达蛋白的活性，将能够正确编码ppt乙酰转移酶的bar基因片段，经过适当的修饰构建入真核表达载体。 - A gus report gene , gained by the method of pcr , was inserted into the pet22b vector between the sites of hindiii and xho i to construct a transitional vector named pet22g then the signal peptide ( pelb leader ) in the pet22g vector was replaced by a containing grb - ast signal peptide fragment to construct expression vector pet22asg which had been transformed into expression host e . coli bl21 ( de3 ) plyss
先将pcr获得的gus报告基因插入到经hind和xho双酶切的pet22b中，构建了中间载体pet22g ；然后再将pcr克隆的一含ast信号肽的片段as取代pet22g上的信号肽pelbleader序列，构建了大肠杆菌分泌表达载体pet22asg 。