克隆方式 meaning in English
clone method
Examples
- Used long pcr primers as sequencing primer , the two sides of sequence of the long pcr product were separately sequenced one reaction by the direct sequencing method of pcr products , and the sequencing sequence were carried on constrasted analysis with sequence of 168 strain in genebank
一2片段克隆质粒为模板,采用skippcr方法,得到了约4 . 3kb产物,以ta克隆方式,插入pgem一t载体中。将经鉴定的阳性克隆质粒进行测序,结果表明: biob与biol基因间的5lbp的终止子序列已被删除。