序列设计 meaning in English
sequential design
Examples
- The results demonstrated that the momps were protective antigens and the momp - iscoms of aeromonas hydrophila could induce the host to mount satisfied immunity . a pair of primers were designed according to the published nucleotide sequence of a putative outer membrane protein gene ( omp ) of aeromonas hydrophila . with the specific primers , a target fragment about 1 . 1kb was amplified from aeromonas hydrophila l316 via pcr . the target fragment was inserted into the linearized pgem - t easy vector
根据已发表嗜水气单胞菌的外膜蛋白基因omp的核苷酸序列设计引物，利用pcr技术，扩增、克隆了嗜水气单胞菌l316的主要外膜蛋白基因( momp ) ，经t a克隆，插入到pgem - t系列载体上，测序分析结果表明momp基因最长的开放阅读框( orf )为1035nt ，编码由344个氨基酸组成，分子量为36kda的主要外膜蛋白质( momp ) 。 - In this research , total rna was extracted from fetal liver by the modified single - step method of acid guanidinium thiocyanate - phenol - chloroform ( agpc ) . based on reported sequence of hsa cdna , specific primers were designed to amplify its encoding region by rt - pcr . at the same time , kozak sequence was added to the translation site , to strengthen initiation of translation
本研究通过改进的异硫氰酸胍-酸性酚-氯仿一步法从人工流产胎儿肝脏中提取总rna ，然后根据报道的序列设计特异性引物，同时在上游引物中引入了kozak序列，经rt - pcr克隆到hsacdna编码区序列，长度为1758bp 。 - The loop sequence of mb1 and mb2 were the anti sense and sense sequence ofing1 , respectively the sequence of mb3 was a piece of ssrna sequence in tobacco mosaic virus , which had no analogical to human gene . mbl was the most suitable probe because mbl had the highest fluorescence enhancemen after hybridizing wtth rna extrated froin normal cell
第三章，根据一种常见的病毒烟草花叶病毒( tmv )的核酸序列设计了分子信标荧光探针，由于tmv的遗传物质是rna ，分子信标又具有很高的特异性和灵敏度，因此感染了病毒粒子的植物叶片在经过简单处理后，可用分子信标检测叶片上。 - Bioassay showed that the second - stage juvenile ( j2 ) could be killed by the raw extract of serine protease . serine protease was characterized after purification with ion exchange chromatography and gel filtration chromatography . the t profile , t stability profile , ph profile , substrate specificity and inhibitor were tested
该酶分子n -端的氨基酸序列为avidtgveashpef ，通过n -端氨基酸序列设计简并引物，提取被毛孢owvt - 1的总rna ， rt - pcr克隆了该酶的成熟肽编码基因( 1000bp ) ，序列分析表明，丝氨酸含量高达12以上。 - Then , a piece of degenerate primer was designed according to the conserved amino acids of glycine betaine abc transporter system glycine betaine - binding protein as a reversed primer . combined with the opuaa - up , a 2 . 3 kb fragment was obtained through pcr . blast result showed a fragment which contained the partial opuaa , the whole opuab and partial opuac sequences were obtained
再次，根据甘氨酸甜菜碱atp转运系统底物结合蛋白的氨基酸保守序列设计下游简并引物，与atp结合蛋白的上游简并引物组合，经pcr扩增获得2 . 1kb的条带，测序后通过blast比较，结果显示获得atp结合蛋白基因的部分序列、通透酶的全部编码序列和部分甘氨酸甜菜碱结合蛋白基因的核苷酸序列。