重组技术 meaning in English
recombinant dna technique dna
recombinant dna technology
Examples
- The pil - 6 cdna fragment was inserted into pgex - 1 ? t plasmid to construct the expression plasmid pgpil - 6 , the recombinant plasmid was digested by bamh i and pst i to identify whether the pil - 6 cdna fragment was inserted into the plasmid in correct orientation , the pgpil - 6 was transformed into e . coli dh5 ? competent cells
用dna重组技术将已克隆的猪il ? 6cdna片断插入pgex ? 1 t质粒，构建了猪il - 6基因的原核表达质粒pgpil - 6 ，转化大肠杆菌，以bamhi酶切筛选、 psti酶切鉴定转化子，获得了含740bp插入克隆基因正确的重组子。 - By recombinant dna techniques , the vp2 gene of gpv hi strain was fused in frame with 6his gene of prokaryotic expression vector pproex - htb . the recombinant expression plasmid of goose parvovirus vp2 gene pproex - htb - vp2 was transformed into e . coli dh5a and induced with iptg . sds - page analysis showed an induced expression product band about 72ku , which correspond to the sizes of vp2 , reported in the literature
利用dna重组技术，将其结构蛋白vp2基因亚克隆至原核表达载体pproex - htb ， iptg诱导后成功表达出与预期大小相符的约72ku的融合蛋白，光密度扫描对表达产物进行初步定量，表明表达产物约占菌体总蛋白的14 。 - With the development of dna recombination technology , there are also some reports about the expression of recombined ifn protein by prokaryotic expression system , but the expressed proteins often consist in the form of inclusion body = the operations about extracting and purifying proteins are very complicated , biological activity of proteins are not high
随着dna重组技术的发展，国内外亦有利用原核表达系统表达重组ifn蛋白的报道，但表达的目的蛋白常以包涵体形式存在，提取纯化操作步骤复杂、蛋白活性不高。 - To the question of chain of command , a new object - oriented method and bpr ( business process rebuilding ) are studied in this dissertation . at the same time , with this method , a system is developed in order to solve the problem how to realize deep information engineering and boost government affair information engineering
本文针对我国质量技术监督中的行政执法问题，采用面向事件的方法，研究行政执法管理过程中流程重组技术，开发了质量技术监督行政执法管理系统，旨在解决政务信息化建设过程中如何从深层实现信息化的理论和技术，推进我国政务信息化的进程。 - The results were as following : 1 . construction and identifcation of recombinant plant expression vector pbi ! 2i - th by dna recombination technology , sweet protein thaumatin gene was cloned into plant expression vector pbii2i . recombinant plasmid pbii2i - th was constructed successfully by enzyme cutting and electrophoresis
甜蛋白thaumatin基因植物表达质粒pbi _ ( 121 ) - th的构建与鉴定利用dna重组技术，将植物甜蛋白thaumatin基因克隆至植物表达载体pbi _ ( 121 )中，通过酶切、电泳，鉴定thaumatin基因已成功构建到植物表达质粒pbi _ ( 121 )中。