序列设计 meaning in English
sequential design
Examples
- Phaa , phab and phac were amplifed from the subclone of pseudomanas sp . producing pha by pcr . the gel electrophoresis analysis showed that the molecular weights of cloned phaa , phab and phac were equal to fragment speculated from three orfs
利用所提取的开放阅读框架的序列设计三对引物，采用pcr技术，从合成pha的亚克隆片段中分离出phaa 、 phab和phac三个基因片段，经凝胶电泳分析表明，所克隆的三个基因分子量大小与推测的三个开放阅读框架中基因片段大小一致。 - However , only a few host factors with clear in vivo function have been identified . by using pcr and 5 " and 3 " race , we were able to clone a homologue ( named ttom1 ) of arabdopsis thaliania host factor gene , tom1 , which supports the replication of tobacco mosaic virus
根据从拟南芥中已经克隆的支持烟草花叶病毒复制的基因tom1序列设计一对特异性引物，用rt - pcr的方法获得番茄同源基因的部分序列，然后利用5 ’ race与3 ’ race方法从番茄中克隆出全长ttom1基因。 - The study on the function and mechanism of phrip1 is important for clarifying how the cell plate and cell wall form in plants . in this study , full length of phrip1 is amplified by pcr and ligated into pks plasmid , then the bait plasmid , peg202 - phrip1 , is constructed . the inseret gene are sure to be translated into the right fusion protein through its sequence . in the yeast two - hybrid system , the bait plasmid ( peg202 - phrip1 ) and a reporter plasmid ( psh18 - 34 ) are introduced into the yeast ( egy48 ) by co - transformation . then cdna library ( which is in pjg4 - 5 ) is screened and two genes are obtained . the two insert gene fragments are sequenced . one of them is plastocyanin , the other is putative photosystem i reaction center subunit ii precursor , both of them are the necessary components of photosynthetic chain
成膜素相关蛋白1 ( phrip1 )是一个含608个氨基酸的蛋白质，它对于植物胞质分裂中细胞板的形成起到了十分重要的作用。研究phrip1的功能和机制，对在分子水平上阐明植物细胞板以及细胞壁形成的机理具有重大的生物学意义。在本实验中，根据phrip1的序列设计引物对其进行pcr扩增，得到该基因后将其连接到了pks质粒上，并进一步构建成了诱饵质粒peg202 - phrip1 。 - The full coding regions of bdnf genes were amplified from the genome of tylototriton taliangensis , phrynocephalus hongyuanensis , japalura splendida and cyclophiops major , respectively by pcr with the primers designed on the sequence of human bdnf gene . the pcr products were cloned into the vector pucis of esherichia coli . sequence analysis showed that the coding regions of three reptiles are the same ( 741 bp ) in length and these bdnf genes encode a peptide of 246 amino acid residues while that of tylototriton taliangensis is 744 bp in length and encodes a peptide of 247 amino acid residues
根据已有的人bdnf结构基因的全长序列设计了一对引物，利用pcr技术分别从大凉疣螈( tylototritontaliangensis ) 、红原沙蜥( phrynocephalushongyuanensis ) 、丽纹龙蜥( japalurasplendida )和翠青蛇( cyclophiopsmajor )的基因组dna中扩增到目的dna片段，并将其分别克隆到大肠杆菌载体中，然后对所获得的阳性克隆进行测序。 - This dissertation , based on the theories of nonlinear dynamics and modem communications , and with an aim to apply the excellent chaos characteristics to the communication system to efficiently improve its performance , focuses its study on chaotic correlation synchronization , enhanced fm - dcsk modulation , optimal chaotic sequences designing , vd - rake receiver and arithmetic separating of chaotic signals and noise
本论文紧紧围绕混沌通信这一主题，以非线性系统理论和现代通信理论为基础，将混沌优良的特性应用于通信系统中，重点研究混沌通信中混沌相关同步、改进的fm - dcsk调制方式、最佳混沌序列设计、 vd - rake接收机以及基于流形分解的混沌信号与噪声的分离算法等关键问题。