重组技术 meaning in English
recombinant dna technique dna
recombinant dna technology
Examples
- These results suggested that the 65kda protein was a degraded product , bioassay result showed that insecticidal activity of bl - 3 was 3 - fold higher than that of bl - 2 against 3rd instar plutelh xylostella . 3 . expression of cryla ( b ) gene in pseudom on a s fluoreseent pfx - 18 in order to study expression characteristics of icps gene in pseudom onas fluorescent , a new simple transformation method of pf was established
3 .用上述重组技术获得了含有刀口基因的lz20和含有尸2拼即“的lz20a克隆株，通过基因的诱导表达，发现p ”和pz浏民有不同的作用机制，证明了p20具有保护cyt人溶细胞的作用，这种作用并不依赖大肠杆菌受休的不同而改变 - In the detection of protocol analysis model , we mainly studied the fragment reassembly of ip packet on the bases study of tcp / ip protocol . in the detection of pattern match model , we analyzed several kinds of pattern match algorithms such as : kmp , bm , bmh and multiple pattern match algorithms ( wang 2002 )
在基于协议分析的检测方法中，从tcp / ip协议族的层次结构出发，主要分析了ip包的分片重组技术；在基于模式匹配的检测方法中，主要分析了多种模式匹配算法： kmp 、 bm 、 bm的改进算法以及多模式匹配算法。 - For example , in 20 centuries 70 time , as the progress of molecular biology , be united in wedlock with project technology photograph , open up biology technology new field : gene recombines technology of cross of technology , pcr technology , dna and technology of protein alignment analysis , element to wait , the birth that promoted gene project , protein project , cell project , enzymatic project to wait and development
例如，在20世纪70年代，随着分子生物学的进步，与工程技术相结合，开辟了生物技术新领域：基因重组技术、 pcr技术、 dna和蛋白质序列分析技术、分子杂交技术等，促进了基因工程、蛋白质工程、细胞工程、酶工程等的诞生和发展。 - However , its wide application in manufacture has always been restricted by such a question as low phytase - producing level of wild strains , so before the wild strains which can produce acidic phytase are widely used , their phytase activities must be improved by various means , in which using an efficient expression system to express heterogenous phytase is a main consideration
植酸酶主要存在于微生物和植物中，动物体内也有少量存在，但真正具有开发价值的主要是微生物产生的植酸酶，尤其是曲霉产生的酸性植酸酶。国外早在1991年就成功地利用dna重组技术获得第一株酸性植酸酶的工程菌，并已投入商品化生产。 - Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells , we design a pair of particular primers , and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic . the expression plasmid was identified with pcr and dna sequencing . pbv220 - r hpf4 was transformed into e . coli dh5a , bl21 ( de3 ) and induced by increasing the temperature to 42 . we identified the expression protein by sds - page and western - blotting
目的1人血小板因子4 ( hpf4 )原核高效表达克隆的构建2重组hpf4的表达及分离、纯化工艺研究3重组hpf4的特性研究方法根据原核细胞表达真核蛋白的基因表达调控特点，设计合成一对特异引物，在pt7 - 7 - rpf4表达质粒的基础上，应用聚合酶链式反应( pcr )对其cdna进行改造，通过dna重组技术构建成重组hpf4原核表达质粒pbv220 - rhpf4 ，用快速pcr检测法、 dna测序分析，鉴定重组hpf4表达质粒的正确性。