binary vector meaning in English
二进制向量
双运载体
Examples
- In the experiment , the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked . it was expressed in e . coli and its protein was determined . after having been properly modified , the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing , which is the prerequisite work for genetic transformation
本实验从抗除草剂转基因bobwhite小麦中，利用pcr克隆的方法扩增出bar基因全长，并在原核表达系统中表达，鉴定表达蛋白的活性，将能够正确编码ppt乙酰转移酶的bar基因片段，经过适当的修饰构建入真核表达载体。 - I have used low copy pbin19 and single copy pmw755i5j binary vectors as backbone plasmids , to create a gene targeting insertion vector designated gfp tnos . after agro - infiltration into transgenic nicotiana benthamiana 16c , progeny were analyzed genetically for phenotypic changes , sirna accumulation , and dna methylation
采用农杆菌浸润法( agro - infiltration )感染转基因本生烟16c ，并对同源基因瞬时表达所引起的植物表型变化、小分子rna的产生、 dna甲基化程度、以及相关性状在后代中的遗传情况进行了检查。 - 2 . an anther specific chimaeric male sterile gene expression box with a enhanced promoter ( ta29 ) driving coda gene was constructed and the expression box was inserted into binary vector p3301 that contains a l - phosphinothricin ( ppt ) - resistant selective marker gene and - glucuronidase ( gus ) reporter gene in t - dna region
以增强的ta29启动子驱动克隆的coda基因，构建成花药特异性嵌合基因表达盒；将此表达盒插入双元载体p3301 ，构建成以ppt抗性基因为选择标记，以gus为报告基因的植物表达载体。 - Is toxic to tenebrio molitor l . . the homological cry3aa gene was modified according to the characters of poplus genes and the other features of eukaryotic gene , and was artificially synthesized . the original and modified homological c / y3aa gene were cloned into binary vector pb121 and pbin438 respectively , and transplanted into 84k clone ( p . tomentos x p . glandulossa ) to breed new anti - insects tree species
通过对氨基酸密码子的偏好、使用频率、多聚腺苷酸信号序列( ppss ) 、 mrna不稳定序列( dst )以及kozak等结构的优化改造，人工设计、合成了预期能在杨树中高效表达的毒蛋白基因modifiedcry3aa同源基因； ( 4 )利用强启动子表达载体pet30 - b完成了cry3aa同源基因在大肠杆菌中的表达。