阅读框架 meaning in English
open-reading frame
reading-frame
Examples
- Phaa , phab and phac were amplifed from the subclone of pseudomanas sp . producing pha by pcr . the gel electrophoresis analysis showed that the molecular weights of cloned phaa , phab and phac were equal to fragment speculated from three orfs
利用所提取的开放阅读框架的序列设计三对引物，采用pcr技术，从合成pha的亚克隆片段中分离出phaa 、 phab和phac三个基因片段，经凝胶电泳分析表明，所克隆的三个基因分子量大小与推测的三个开放阅读框架中基因片段大小一致。 - The 3 . 4kb ecori dna fragment of the pgxn201 and the mutant ecori dna fragments of the three mutant plasmids were subcloned into the ecori sites of the clone vectors ml3 and the pgem3z - f ( + ) respectively . through dna sequencing and dna sequence analysis by comparing with the bradyrhizobium japonicum dna sequences reported , we found that there are four intact open reading frames in the 3 . 4kb fragment and it was highly homologous to the bradyrhizobium japonicum dna sequence reported , and the tn5gusa5 containing in the pgxn217 was inserted in one of the four open reading frames . this open reading frame located on about 5 . 5kb upstream of the nfec gene , which includes 207bp nucleotides , coding 68 putative amino acids and this is completely identical to that of the bradyrhizobium japonicum dna sequence reported
本研究首先利用转座子tn5gusa5对重组质粒pgxn201进行诱变，获得3 . 4kbecori片段插入了tn5gusa5的突变质粒pgxn215 、 pgxn216 、 pgxn217 。对这些突变质粒进行亚克隆及测序分析并与基因库中已报道的慢生型大豆根瘤菌的dna序列作比较，发现突变质粒pgxn217中tn5gusa5恰好插入在一个未知功能的开放阅读框架内。将pgxn201的3 . 4kbecori片段与m13作连接，获得亚克隆pgxn201c ，对pgxn201c进行测序分析，发现与基因库中已报道的慢生型大豆根瘤菌的dna序列有95的同源性。 - The cdna is 2 149 bp long with an open reading frame of 1 697 bp , which encodes a polypeptide of 565 amino acids , preprotein of 62 . 3 kda ; 5 " untranslated regions contains 59nt , 3 " untranslated regions contains 391nt , a poly ( a ) tail signal and long poly ( a ) tail
该cdna全长2149bp ，包含一个1697bp的开放阅读框架，编码565个氨基酸。在起始密码子上游有一个由59个碱基组成的5 ’非编码区；在终止密码子下游有一个由391个碱基组成的3 ’非编码区，包括4个分解信号、 1个加尾信号和1个长度为17个腺苷酸的poly ( a )尾。 - In this paper , first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv . the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector . after transforming e . coli dh5 a , ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr . presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc . comparing the aquired sequence of 3abc with that of reference strains , the homology is more than 99 percent . the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo . lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene , which happened to form a terminator codon behind 3ab gene , but it contained the complete open reading frame ( orf ) of 3ab gene . positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ) , bacteria were detected by sds - page and western blotting after properly treated . the results showed that the 3ab gene expressed successfully in e . coli and 33 . 5ku fusion protein can be recognized by the positive bovine serum of fmdv . the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
扩增产物连接到pgem - teasy载体中，转化大肠杆菌dh5菌株，筛选氨苄青霉素抗性菌落，提取质粒经酶切鉴定、 pcr分析以及确证性测序证明，所克隆的1500bp左右的片段含有完整的3abc基因，与国外参考序列相比，同源性在99以上。将重组质粒pgem - 3abc和表达载体ptriex - 4neo分别用sal和bgl与xho和bgl消化后，亚克隆3abc基因至原核表达载体ptriex - 4neo中，通过酶切鉴定、 pcr扩增以及序列分析，发现克隆到ptriex - 4neo载体上的片段于3abc基因708bp处出现了17bp的缺失，碰巧在3ab基因后形成一终止密码子，但3ab基因的阅读框架完整，选出含有3ab基因完整阅读框架的阳性克隆，用iptg诱导表达，收集菌液进行sds - page电泳、 westernblotting分析，结果表明， 3ab基因在大肠杆菌中成功表达，其表达产物为分子量33 . 5ku的融合蛋白，并能被口蹄疫病毒阳性血清识别。经薄层扫描分析，表达量占总蛋白量的26以上。 - In this research two full - length cdnas have been cloned by a combination of rt - pcr and 3 " - is1 - race with synthesized degenerate primers from young leaves of vicia faba l . , pichia methanolica high - level expression systems of the genes have been constructed , and the milligram expressed protein was purified using probond resin purification system , which may result in further identification of the function of the aba binding protein . the full - length cdna of abp370 fragment is 3449 bp long and has an open reading frame of 2304 bp encoding 768 amino acids with 876 bp long 5 ' - utr , 369 bp long 3 ' - utr and poly ( a ) tail . the full - length cdna of abp640 fragment is 1012 bp long and has an open reading frame of 780 bp encoding 260 amino acids with 88 bp long 5 ' - utr , 144 bp long 3 " - utr and poly ( a ) tail
3 - 5 - race扩增片段序列分析结果表明， abp370扩增片段的全长cdna为3449核苷酸，其中5非翻译区为876个核苷酸， 3非翻译区为369个核苷酸并末端带poly ( a )尾巴，从起始密码子atg至终止密码子tga ，含有一个编码768个氨基酸残基的开放阅读框架( 2304bp ) ； abp640扩增片段的全长cdna为1012核苷酸，其中5非翻译区为88个核苷酸， 3非翻译区为144个核苷酸并末端带poly ( a )尾巴，从起始密码子atg至终止密码子taa ，含有一个编码260个氨基酸残基的开放阅读框架( 780bp ) 。