阅读框架 meaning in English
open-reading frame
reading-frame
Examples
- Pcr product was cloned to the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector , and e . coli bl21 was transformed by the recombinant plasmid for expression
Pcr产物经纯化、酶切后，按正确的阅读框架定向克隆到表达性载体pgex - 6p - 1中谷胱甘肽转移酶( gst )基因的下游。 - Human gnt - v contains 741 amino acids with six potential sites for n - glycosylation and bears high homology to gnt - v of rat . its gene is located on chromosome 2q21 containing 17 exons . gnt - v protein is encoded by exons 2 - 17 as open reading frame
人类gnt - v由741个氨基酸组成，有6个潜在的n -糖基化位点，基因定位于染色体2q21 ，含有17个外显子，其开放阅读框架由外显子2 - 17进行编码。 - After sequencing of 84 cdna clones and removing redundant cdnas , we obtained 36 cold - regulated unique cdna clones . 12 cdna clones were expected to be novel genes , because no sequence homology with any known sequences was found in genbank databases
全长基因ej175共有603bp ，对其可读阅读框架进行分析，从57 - 515位核苷酸的一段序列，包含了起始密码子和终止密码子，编码152个氨基酸的多肽。 - The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest . then the recombinant plasmid was identified by endonuclease analysis , pcr ampliation and dna sequencing . the report showed that the recombinant plasmid had right open reading frame
重组质粒经酶切鉴定， pcr鉴定和测序，结果证实猪肺炎支原体黏附因子p97基因的抗原决定簇r1区定向插入了质粒pet - 32a ( + ) ，且阅读框架正确。 - Dna sequencing of the appa gene showed an open reading frame of 1299 bp . the deduced appa phytase composed of 432 amino acids ( predicted molecular mass , 47 . 06 kd ) also contained the reserved active - site motif rhgxrxp , which is shared by other phytases and acid phosphatases
测序结果显示appa基因阅读框架为1299bp ，编码432个氨基酸，编码产物理论分子量为47 . 06kd ，同时它也具有其它植酸酶与酸性磷酸酶的活性保守基序rhgxrxp 。