连续传代 meaning in English
continuous passage
Examples
- The vaccine was developed by passaging eiav strain liaoning ( eiav l ) in donkeys in vivo , obtaining a donkey - adapted virulent equine infectious anemia virus ( eiav da ) and then passaging it in donkey leukocyte cultures for more than 120 times in vitro
该毒株是将eiavl株（ eiavl ）通过驴体传代，使其毒力明显增强，然后在驴白细胞培养物上连续传代致弱获得的。 - Cells were collected after being passaged 5 times and used to identify infection by rt - pcr , direct fluorescent antibody , sandwich elisa . results indicated that integrated particles of csfv could be obtained from constructed full - length cdna
连续传代5代以后，收集各w摘要代细胞至第10代，采用rt一pcr 、直接荧光抗体染色和夹心elisa技术进行鉴定，结果均为阳性。 - Results : the neural stem cells isolated in newborn rats had the potential of forming clones and serial passage , expressing neuroepithelial stem cell protein ( nestin ) and differentiating into neurons and astrocytes and oligodendrocytes
结果：新生大鼠脑组织分离的细胞具有连续传代和增殖能力，表达神经上皮干细胞蛋白，诱导分化后的细胞表达神经元细胞、星形胶质细胞、少突胶质细胞特异性蛋白。 - At last , three strains of high activity was induced mutagenesis by ntg , after the routine screening procedures , activity of n28 and n118 was higher 47 . 5 and 40 . 9 percent than that of a . niger an01001 , and higher 29 . 5 and 23 . 7 percent than that of a199
最后，用亚硝基胍对产酶较高的3个菌株诱变，经复筛后， n28和n118的酶活比黑曲霉an01001高出47 . 5和40 . 9 ，比a199高出29 . 5和23 . 7 ， n28和n118经过5次连续传代后，产酶能力基本稳定。 - According to these problems , we adopt to the method of mending material , optimize to fermentation media and partly ferment condition . finally , we excogitate a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified . with the plasmid pbv220 - ifnr , pbv220 - hgfa , pbv220 - hgfb , pbv220 - hpk5 that expresses serve as the model , adopting the biostat - c15l of b . braun company , utilize the method of mending material to ferment , through optimization fermentation media and optimization partly ferment condition ( ventilate quantity , stir speed , mend material speed ) , eventually establishment a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified
以我室构建并稳定表达的重组质粒pbv220 - - ifn 、 pbv220 - hgf 、 pbv220 - hgf 、 pbv220 - hpk5为模型，分别从不同的表达宿主菌中筛选出一种适合大规模生产的菌种bl21 ( de3 ) ，该工程菌株连续传代100代表达质粒不丢失，表达量稳定；采用b . braun公司的biostat - c15l自控发酵罐，运用分批补料技术分别进行四种工程菌的高密度发酵，通过优化工程菌发酵的培养基配方及优化部分发酵条件(通气量、搅拌速度、补料速度) ，最终建立一种适于目的基因高效表达的高密度发酵工艺模式。