序列设计 meaning in English
sequential design
Examples
- Two pairs of primers were designed based on the sequence of mddl to get a 0 . 9kb and an 1 . 1kb pcr products which were confirmed to be expected sequence from mc2155 by dna sequencing
根据该基因的序列设计了两对特异引物，以mc ~ 2155dna为模板，扩增得到0 . 9kb和1 . 1kb的产物。其中的0 . 9kb产物经测序验证与mddl的序列一致。 - Two oligonucleotides were synthesized according to the sequence of p . furiosus extracellular a - amylase gene amya through the genbank and used as primers for pcr with p . juriosus genomic dna as the template
根据genbank公布的p furiosus的-淀粉酶基因amya序列设计两条引物，以p furiosus的基因组dna为模板进行pcr 。 - Two special primers of race were designed based on the above sequence of the rt - pcr product . two fragments of 5 ' cdna ( 895bp ) and 3 ' cdna ( 581 bp ) were amplified by race reaction
以此特异片段设计特异引物，采用race技术分别获得-葡萄糖苷酶cdna3 ’端581bp和5 ’端895bp片段，后再依据上述两段序列设计一对引物，扩增出酶的全长序列1475bp 。 - Which shared 10 % ~ 73 % identities to other rips from plants and 37 % ~ 73 % to other rjps from cucurbitaceae . six novel rip gene fragments ( 408 ' bp ) , 1 from benincasa hispida and 5 from cucurbit a moschaia
根据葫芦科rip上、下游两段高度保守的氨基酸序列设计简并引物对ly1 ly2 ，对基因组dna进行pcr扩增，首次建立了rip新基因快速筛选体系。 - In this study , an escherichia coli strain with high phytase activity was screened from pig excreta . using primers designed according to the sequence originally described by dassa , appa gene was amplified by pcr technique
本实验从猪粪中筛选出一个高产植酸酶的e . coli菌株，根据dassa等报道的序列设计一对引物通过pcr方法扩增了appa基因。