| 1. | The method of polyamide column chromatography is preferred for purifying ag , based on the comparisons of yield , purity and cost
综合产品得率、糖含量以及成本等多方面因素，选择直接用聚酰胺层析柱来纯化阿拉伯半乳聚糖较为合适。 |
| 2. | The expressed proteins were purified by ni2 + chelation affinity chromatography . the recombinant proteins were prepared with the purity more than 95 %
用ni ~ nta亲和层析柱进行纯化，获得1 ’ rka膜外域各结构域重组蛋白，纯度均在95 %以上。 |
| 3. | The amount of the goal protein was evaluated by densitometric scanning . it indicated that the product of the vp2 gene was 14 % of total bacterial protein of dh5a
表达产物经ni - nta金属鏊合层析柱纯化后，得到了纯度较高的6his - vp2融合蛋白。 |
| 4. | The amount of the goal protein was evaluated by densitometric scanning . it indicated that the 45ku product of the vp6 gene was 26 . 5 % of total bacterial protein of bl21
表达产物经ni - nta金属螯合层析柱纯化后，得到纯化的his - taggedvp6融合蛋白。 |
| 5. | The purification effects by the two methods were compared , showing that the yield of arabinogalactan purified by the resin was 68 . 28 % , and the sugar content was 95 . 02 % , while those by polyamide column were 75 . 67 % and 98 . 30 % respectively
结果表明，经大孔树脂层析柱纯化后的阿拉伯半乳聚糖产品得率为68 . 28 % ，糖含量为95 . 02 % ；经聚酰胺层析柱纯化后的产品得率为75 . 67 % ，糖含量为98 . 30 % 。 |
| 6. | With ni2 + - lda - sepharose , the cbd tag was removed . according to the results of sds - page and western blot analysis , the product of outflow was the target protein lt a 27 . the purity of lt a 27 was about 95 %
运用niz +金属鳌合亲和层析柱进一步快速纯化去除融合头，上样流出经sds一队ge分析和western印迹分析ii1 :明得到的纯化产物为淋巴毒素缺失体蛋白lt凸27 ， lt 2 :产物纯度可达95 %以上。 |
| 7. | This material was loaded onto a silica gel column and the column was eluted with chloroform several times until the fraction of interest was collected . finally we obtained some light yellow green ointment liquid f i
采用硅胶作干层析柱填充物，用氯仿作展开剂和洗脱液，反复硅胶层析，对柄海鞘鞘囊氯仿浸提物进行分离、提取与纯化，最后得到一浅黄绿色的油状液体fraction (以下均称f ) 。 |
| 8. | Macroporous adsorptive resin column and polyamide column were used respectively to purify arabinogalactan ( ag ) from larix gmeini rupr . . the contents of impurity in ag and sugar varying with time were analyzed
摘要分别采用大孔吸附树脂柱层析和聚酰胺柱层析技术纯化兴安落叶松中的阿拉伯半乳聚糖，研究了两种层析柱洗脱过程中的杂质含量、糖含量随时间变化的情况，对比分析了它们对阿拉伯半乳聚糖的纯化效果。 |
| 9. | But these techniques can not provide physiological ph and ionic strength , and radio - iodination of heparin required for ace endangers health of human and environment . for avoiding the radiological hazards and reflecting directly the binding characteristic of heparin to protein
但亲和层析柱易被污染失活， ace技术不能提供生理ph范围和离子强度及ace技术必需的标记肝素的放射性物质对人和环境具有很大危害。 |
| 10. | Blood and urine with water or the supernatiant of viscera sample being deproteined with 6 % hclo4 solution was add 0 . 2ml gdx301 porous polymer bead and shaken to absorb the drugs in specimen . then the bead was packed into a glass column . the column was washed with 10ml water and elution was effected with dichloromethane
血、尿用水稀释，脏器用6高氯酸沉淀蛋白后，加0卫dgdx301树脂振摇，滤集树脂于小层析柱中，用水10inl淋洗，二氯甲烷洗脱，收集洗脱液sml ，挥干后用0 |
