| 1. | The supernatants were dialyzed against 50 mm phosphate buffer ( pb ) ( ph 6 . 5 ) , and passed through a blue - sepharose 6b column ( 3x30 cm )
Iptg诱导表达目的蛋白，上清液经sds一page电泳后看到约32kd的目的条带。 |
| 2. | After absorbed with protein g - sepharose beads to remove the igg antibody , the pooled sera were then used for immunoscreening of the cdna library of schistosoma japonicum adult worms
蛋白同源性分析显示， sj43b编码蛋白与来自人和、鼠的含洲3结构‘域蛋白的同源性为3洲。 |
| 3. | The cytotoxicities of rta and the fusion toxin rta - yqrl were measured by the mtt assay in hela , skov - 3 , and wish cells following fluid - phase endocytosis . [ methods ] 1
因为rta可与f3ga发生特异性结合，我们用bule一sepharose一6b柱对rta和rta一yqrl蛋白分别进行亲和层析纯化。 |
| 4. | After dialysis , the recombinant gst - ri was purified by rnase a - sepharose 4b affinity chromatography , and the single band of gst - ri was showed on a sds - electrophoresis gel to remove the extra urea
复性的gst一班经knasea - sepharose4b亲合层析纯化， sds一page鉴定得到单一的gst一rj条带。 |
| 5. | By gel filtration on sepharose cl - 6b and cellulose acetate pellicle electrophoresis test , hpa and hpb were respectively a single strait symmetry apex and the result of electrophoresis assume a single spectrum strip
经sepharosecl - 6b柱层析和醋酸纤维素薄膜电泳检验， hpa和hpb均为单一狭窄对称峰且电泳呈单一谱带。 |
| 6. | The purification of this halocin was achieved by combination of tangential flow filtration ( tff ) , sephadex g50 and deae - sepharose chromatography . the n - terminal amino acid sequence was also determined by edman degradation
Halc8对大多数的极端嗜盐古菌(包括部分嗜盐碱古菌)有抑制作用，但对细菌则无明显的抑制作用。 |
| 7. | Sepharose affinity chromatography . the apparent molecular weight of purified ghrp - scu - pa - 32k was 33kd by sds - page and mass spectrometry and its specific activity was 150000iu mg protein , according to fibrin plate determination
经锌离子螯合亲和柱纯化的产物， sds - page显示为单一蛋白条带，分子量为33kd ，质谱法测定分子量也为33kd 。 |
| 8. | After the lysate was purified with gst sepharose 4b affinity chromatography , a specific band of 34kd appeared in sds - page gel , the purity and the titer of the antibody against fusion protein gst - hnadc3 could reach up to 90 % and 1 : 32 , respectively
菌体裂解液经gst亲和层析纯化后， sds page上出现一分子量为34kd的特异蛋白条带，纯度可达90 。 |
| 9. | In order to attain bioactive glycoprotein , glycoprotein of the leaves of camellia sinensis , was purified from coarse glycoproteins purified by sephadex g - 100 gel chromatography , by cona - sepharose 4b affinity chromatography
为了纯化天然糖蛋白，进行了茶树叶糖蛋白的cona - sepharose4b亲和层析，从sephadexg - 100凝胶过滤收集的粗糖蛋白中，分离茶树叶糖蛋白。 |
| 10. | Then the product was purified by glutathione sepharose 4b chelation affinity chromatography , and upper purified gst - gnrh / trs fusion proteins was received for further the foundations established in the scientific research and the real application
表达产物经谷胱甘肽琼脂糖4b亲和层析纯化后，得到了纯度较高的gst - gnrh trs融合蛋白。为进一步科研和实际应用奠定基础。 |
