| 1. | The modeling indicates mlap has a slot to catalyze the reaction . the hydrogen bond is important to the cold - adaptation of mlap
模建的结果表明此蛋白酶含有一个狭缝，底物就在此处进行催化水解反应。 |
| 2. | The mlap was analyzed with bioinformatics software . it has two domains : zincins catalytic domain and c - terminal domain of metalloprotease
该酶含有两个结构域，分别为zincins催化结构域和金属蛋白酶羧基端结构域。 |
| 3. | The mlap was modeled with different methods , then analyses the model with software . the result indicates that the geno3d model is good in stereochemistry and biology function
结果表明用geno3d预测方法得到的结果比用其它方法的模型更符合立体化学特性和生物学功能的规律。 |
| 4. | Marine low - temperature alkaline protease ( mlap ) is a kind of extracellular enzyme from a kind of marine bacterium of qd80 . the study mainly concerned about gene engineering bioinformatics and homology modeling of mlap
海洋低温碱性蛋白酶( marinelow - temperaturealkalineprotease ， mlap )是由一海洋杆菌? qd80产的一种胞外酶。 |
| 5. | One positive clone was obtained from the libraries with casein flat and electrophoresis comparison method . analyzed the sequencing result , the positive clone include the open read frame of mlap gene and upper regulatory sequence
序列测定分析表明，此重组质粒包含有长度为1377bp低温碱性蛋白酶基因的完整的开放读码框架( orf )和上游基因调控序列。 |
| 6. | Studied the pl molecular weight histidine of catalytic group of mlap and the influence of zn2 + ca2 + and edta to enzyme activity for validation the results of bioinformatics analysis and homology modeling . the cold - adaptation is validated by the catalysis of mlap
为了验证碱性蛋白酶的生物信息学分析结果和模建的结果，我们对其等电点、分子量、催化性基团组氨酸以及锌离子、钙离子和edta的影响进行了实验分析。 |
