| 1. | Construction and expression of recombinant human thymosin fusion gene in pichia pastoris 在毕赤酵母中的表达 |
| 2. | Construction of p21 egfp fusion gene expression vector and its expression in hrpe cells 15细胞乙型肝炎病毒复制的实验研究 |
| 3. | Construction and characterization of stable 1 - integrin - gfp fusion gene overexpressing hcc cell lines 融合蛋白稳定过表达肝癌细胞系的建立 |
| 4. | Construction and expression of recombinant eukaryotic expression plasmid of ubiquitin and epstein - barr virus nuclear antigen 1 fusion gene 病毒核抗原1的融合表达载体的构建及表达 |
| 5. | Gfp expression was monitored using a fluorescence microscope . the result showed that the fusion gene was expressed at a low level 利用脂质体转染的方法在美国棉铃虫细胞hz中表达了gfp - actin融合基因。 |
| 6. | Put this fusion gene under the control of the promoter of ie1 gene , and then an eucaryotic cell expression plasmid vector pgem / ie1 " - gfp - actin resulted 将该融合基因置于ie1基因启动子的控制之下,构建成了真核表达质粒pgem ie1 - gfp - actin 。 |
| 7. | On the base of construction of pbi121vp7 , we constructed the fusion gene expression vector pbi121ctbvp7 by the same sigle cloning site ( ndei and xbai ) of vector puc19ctb and pbi121vp7 设计具有ndei和saci单限制性酶切位点的vp7基因引物,通过pcr扩增出vp7基因并经测序验证。 |
| 8. | Recombinant plasmids pgfpexpa that containing cry3apro - gfp fusion gene and pgfpexpb that containing btl - bthpro - gfp fusion gene were transformed into e . coli and b . thuringiensis plasmidless strains , respectively 该质粒的dna序列已被genbank不11genome收录,序列号分别为af202532不11nc001272 。 |
| 9. | By genetic engineering methods , ureb gene and ureb - hspa fusion gene were amplified by pcr and cloned into a prokaryotic expression plasmid ptrc99a - asd , and identified recombinant plasmid was then 口服、鼻饲免疫balb / c小鼠,在肠液和血清中可以分别检测到针对hpylori的特异性分泌型iga和址g抗体。 |