| 1. | The monopartite genome of tobacco mosaic virus is a plus-strand rna molecule of 6395 nucleotides .
烟草花叶病毒的单份基因组是一个6395个核甙的（）链RNA分子。 |
| 2. | Studies on virus resistance of transgenic tobacco with tmv replicase gene
转烟草花叶病毒复制酶基因烟草的病毒抗性研究 |
| 3. | Spread characteristics of tobacco mosaic virus through clipping in tobacco floating - seedling system
烟草漂浮育苗剪叶传播烟草花叶病毒的特点 |
| 4. | The monopartite genome of tobacco mosaic virus is a plus - strand rna molecule of 6395 nucleotides
烟草花叶病毒的单份基因组是一个6395个核甙的（ ）链rna分子。 |
| 5. | 3 . arabidopsis plants were transformed with the constructed plant binary expression vector . eighteen independent antibiotic - resistant arabidopsis transformants for dreb1c were obtained by using a vacuum infiltration method
载体全长为13kb ，有一个烟草花叶病毒35s强启动子和nos终止序列，利用bamhi和sacl做为酶切位点，构建载体。 |
| 6. | However , only a few host factors with clear in vivo function have been identified . by using pcr and 5 " and 3 " race , we were able to clone a homologue ( named ttom1 ) of arabdopsis thaliania host factor gene , tom1 , which supports the replication of tobacco mosaic virus
根据从拟南芥中已经克隆的支持烟草花叶病毒复制的基因tom1序列设计一对特异性引物，用rt - pcr的方法获得番茄同源基因的部分序列，然后利用5 ’ race与3 ’ race方法从番茄中克隆出全长ttom1基因。 |
| 7. | The loop sequence of mb1 and mb2 were the anti sense and sense sequence ofing1 , respectively the sequence of mb3 was a piece of ssrna sequence in tobacco mosaic virus , which had no analogical to human gene . mbl was the most suitable probe because mbl had the highest fluorescence enhancemen after hybridizing wtth rna extrated froin normal cell
第三章，根据一种常见的病毒烟草花叶病毒( tmv )的核酸序列设计了分子信标荧光探针，由于tmv的遗传物质是rna ，分子信标又具有很高的特异性和灵敏度，因此感染了病毒粒子的植物叶片在经过简单处理后，可用分子信标检测叶片上。 |
| 8. | The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector . the sequencing results showed that pap gene had 99 . 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ) . the iptg - inducible expression vector containing the pap gene was constructed and transferred into e . coli bl21 ( de3 ) - plyss
将缺失型pap基因克隆到植物表达载体pbi121中，通过液氮冷冻法将重组质粒转入农杆菌lba4404细胞中，然后采用叶盘法，在该农杆菌的介导下将pap基因导入普通烟草中，经过卡那霉素抗性筛选，最后获得了转pap基因的工程烟草植株，摩擦接种烟草花叶病毒( tmv ) ，与非转基因烟草相比，能够推迟症状表现达2月之久，说明pap基因能够在其它植物体内产生有活性的高抗病毒的蛋白质。 |
