鸡传染性贫血病毒 meaning in Chinese

cav

Infectious bursal disease virus has been a great concern for the poultry industry for a long time , particularly for the past decade when its " re - emergence " in variant or highly virulent forms . in this study , two sets of primers ( pta and pts , ibda and ibds ) , flanking the hyper - variable region of vp2 gene , were designed to run a reverse transcription polymerase chain reaction ( primary rt - pcr ) and nested - pcr . both of these assays can amplify all of 12 reference strains which including pathotypes cibdv , vvibdv and vibdv , but not the 5 negative reference pathogens of chicken
结果12个参考毒株均能扩增出约679bp的目的片段，而阴性对照的常见5种鸡病病原：鸡新城疫病毒( ndv ) 、鸡传染性支气管炎病毒( ibv ) 、鸡传染性贫血病毒( caiv ) 、大肠杆菌和多杀性巴氏杆菌均没有扩增到任何片段；应用建立的技术对疑似ibd的34份临床病料进行检测，并同时在基础rt - pcr扩增的片段内设计另一对引物进行nested - pcr 。