非变性聚丙烯酰胺凝胶电泳 meaning in Chinese
native polyacrylamide gel electropho-resis
nondenaturing polyacrylamide gel e-lectrophoresis
nondenaturing polyacrylamide gel electrophoresis
Examples
- Method to collect respectively 180 unrelated males " venous blood 500ul , who lived in shanxi province , 120 unrelated mongolians " venous blood 500ul , who lived in the inner mongolia autonomous region , and the blood is anticoagulant with edta , then to extract dna by using the method of phenol - chloroform after ingested by proteinase k at 56 and amplify the dys413 site by using pcr
方法采集180例山西汉族和120例内蒙古蒙古族男性无关个体静脉血各500ul ， edta抗凝，用tkml液反复洗涤至无色，加入2蛋白酶k缓冲液180ul ，蛋白酶k ( 20mg ml ) 20ul ，在56消化至液体清亮为止，用酚-氯仿法抽提dna ， pcr扩增dys413位点， 6非变性聚丙烯酰胺凝胶电泳， 1硝酸银染色分型。