阴离子交换层析 meaning in Chinese
anion exchange chromatography
Examples
- Methods 1 mr1 hybridoma was cultured in hollow fibre system . culture supernatant was harvested , then precipitated by half - saturated ammonia sulphate . mfm was purified by deae - sephacel ion exchange chromatography . mri concentration was measured by elisa
硫酸钱半饱和沉淀， oeae阴离子交换层析分离纯化抗co40l单克隆抗体mri 。 pbs透析后， 505一队ge检测纯化抗体的纯度， eusa测定抗体浓度。 - Fusion expression of m - centrin in e . coil bl21 was performed by induction of fptg . fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography . the final products were checked by sds - page gel
融合蛋白gst - m - centrin菌体经过超声波裂解后得到的上清夜经过gst亲和层析后用prescissionprotease ( ppase )酶切，酶切产物再次经过gst亲和层析和hitrapq阴离子交换层析两步柱层析纯化后，得到纯度较高的的m - centrin 。 - The xerocomus spadiceus lectin , xsl , was isolated from extracts of fruiting bodies of the mushroom xerocomus spadiceus using a procedure that involved ( nh4 ) 2so4 precipitation , anion exchange chromatography on deae - cellulose , affinity chromatography on affi - gel blue gel , cation exchange chromatography on cm - cellulose , and gel filtration by fast protein liquid chromatography on superdex 75
从砖红绒盖牛肝菌( xerocomusspadiceus )子实体粗提物中，经过deae -纤维素阴离子交换层析、 affi - gelbluegel亲和层析、 cm -纤维素阳离子交换层析和superdex75fplc凝胶过滤，纯化了砖红绒盖牛肝菌凝集素。