链可变区 meaning in Chinese
gly4ser
Examples
- This is important in attempting to use antibodies as anti - tumor reagents , for example
基因为日本血吸虫单克隆抗独特型抗体np30轻链和重链可变区基固。 - The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library . one of positive scfv clones , named pcsal , was selected with phage - elisa after panning and screening by bull sperm three times . scfv fragment , amplified from pcsa1 , was ligated to pmd18 - t vector for sequencing analysis
取阳性重组噬菌体抗体克隆株pcsa1 ， pcr扩增其scfv基因，筛选重组子进行序列测定，发现其序列符合小鼠抗体基因的一般特征，并且与几株抗磷酸胆碱的抗体重链和轻链可变区序列的同源性达80以上；推测pcsa1scfv针对的抗原是磷酸胆碱类物质。 - Thus , immunologists have sought smaller molecules with the antigen - recognition capability of antibodies . the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable . it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues
二、日本血吸虫单克隆杭独特型抗体np30的单链狐（ scf ）的构建、表达及对balbic小鼠诱导保护性作用研究l 、日本血吸虫单克隆杭独特型抗体np0的单链抗体归cfv ）的构建、表达通过pcr方法体外扩增并经测序验证的重链、轻链可变区（ vh 、 vl ）基因先后重组入原核表达质粒ptha90相应的位点上，中间通过一连接肽（ gly在er ） 。 - Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library . the products were cloned into puc19 vector and their sequences were analysed . the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region , with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv
方法以从噬菌体抗体库中筛选获得的抗hbsag的fab抗体基因为模板，分别扩增出其轻、重链可变区( v _ l 、 v _ h )基因，通过重组pcr方法将轻、重链可变区基因用连接肽( gly _ 4ser ) _ 3的编码序列连接，并引入前导肽编码序列，构建具有l - v _ h - linker - v _ l结构的单链抗体基因。 - In the present study , the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis . total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies . after obtained using rpas system , vh and vl genes were used to assemble scfv gene fragment with a linker primer
应用重组噬菌体抗体库技术，从分泌小鼠抗牛精子sp18抗体的杂交瘤细胞系中分离总rna ，克隆抗体重链和轻链可变区基因，加入连接肽引物( linkerprimer )组装成单链抗体scfv ( singlechainfragmentvariable )基因并用rs引物进行扩增， sfi 、 not酶切，回收后与pcantab5e载体相连，转化e . colitg1宿主菌，构建单链抗体文库。