野油菜黄单胞菌 meaning in Chinese
xanthomonas campestris pv. pelargonii (xcp)
Examples
- A mutant library of xanthomonas campestris pv . campestris ( hereafter xcc ) strain 8004 with 17820 clones was constructed by random transposon tn5gwsa5 mutagenesis , which cover 1800 predicted orfs of xcc genome
用转座子tn5gusa5诱变野油菜黄单胞菌( xanthomonascampestrispv . campestris以下简称xcc ) ，构建了xcc8004tn5gusa5插入突变体库。 - The routine method for inducing protein function evolution is imitating and speeding protein gene mutation . in this research - amylase gene was mutated in vitro by pcr with dntp in which deoxythymidine triphosphate ( dttp ) was partially replaced by 5 - bromo - 2 ' - deoxyuridine - 5 ' - triphosphate ( 5 - bdu ) . the higher the concentration is , the stronger its inducing ability is in a certain range
本研究采用5 -溴脱氧尿苷三磷酸( 5 - bdu )部分取代脱氧胸苷三磷酸( dttp ) ，在pcr的过程中对克隆的野油菜黄单胞菌的-淀粉酶基因进行了体外诱变，诱变结果表明： 5 - bdu浓度越高，诱变越强；浓度越低，诱变越弱。 - 3 . the cloning of a - amylase gene : the methods of shotgun , pcr and rt - pcr were selected to clone a - amylase genes from bacillus subtilis hn503 , xanthomonas campestris pv . campestris 8004 and aspergillus oryzae , hn504 the recombinant plasmid with cloned gene was designated as phn504 , phn503 and phn8004
本研究分别选用了鸟枪克隆法、 pcr和rt - pcr克隆法，成功地克隆到枯草芽胞杆菌( bacillussubtilis ) hn503 、野油菜黄单胞菌( xanthomonascampestrispvcampestris ) 8004和米曲霉( aspergillus口尽留‘ ) hn504的价淀粉酶基因。