重组率 meaning in Chinese
recombination percent
recombination rate
Examples
- By estimating and testing the recombination fraction , it searches the evidence of linkage between a known marker system and a putative gene for a disease
通过估计并检验重组率，连锁分析寻找已知的标识系统和待推定的疾病基因座之间的连接证据。 - Linkage analysis plays an important role in gene mapping . the foundation : the two gene locuses which locate on the same chromosomal ( eg . disease gene and marker gene ) happen to cross over and recombine . the farther the distance between two locuses is , the higher the probability happening to cross over is , the lower the probability that the two locuses are inherited to offspring together is , that is , the degree of linkage is not strong . so we can estimate the distance and the degree of linkage by the recombination fraction between the two locuses to locate gene
连锁分析是基因定位主要策略之一，其基本原理是位于同一染色体上两个基因位点(例致病基因与标记基因)在减数分裂的过程中会发生交换与重组，染色体上的两个位点间距离越远，发生重组的概率就越大，两个位点在一起传给后代的机会就越少，即连锁程度弱，这样由标记位点与疾病位点间的重组率可估算出两者间的距离以及连锁程度，达到基因定位的目的。 - Currently , the research methods can be classified into two main classes , that is , genetic linkage analysis and linkage disequilibrium analy - sis . the two methods explore recombination fraction and linkage disequilibrium coefficient correspondly which are used to measure genetical characters , moreover , statistical methods are used to execute gene mapping
基因定位是人类基因组计划( hgp )重要目标之一，目前研究方法主要分为两大类：基因连锁分析与连锁不平衡分析。两种方法分别借助衡量遗传性质的重组率与连锁不平衡系数，并利用统计方法来进行基因定位。 - Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2 . construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr . the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e . co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重组噬菌体抗体库的构建及鉴定从培养的mg _ 7杂交瘤细胞中提取并分离mrna ，反转录成cdna ；利用pcr分别扩增mg _ 7单抗的重链及轻链可变区基因，并通过? dna连接子将二者连接起来形成mg _ 7单链抗体基因；将mg _ 7单链抗体基因插入pcantab5e ；将连接产物转化感受态tg1大肠杆菌，制备细菌形式的mg _ 7重组噬菌体抗体库；通过菌落计数和限制性酶切分析( ecor和hind )评估mg _ 7重组噬菌体抗体库的容量和重组率。