重复扩增 meaning in Chinese

repeat amplification protcol

2 . 4 preparation of yeast telomerase , the activity of telomerase was examined by the method of trap , polyacrylamide gel electrophoresis and silver staining
2 . 4酵母端粒酶的制备，端粒重复扩增法( trap ) 、聚丙烯酞胺凝胶电泳及硝酸银染色法检测端粒酶活性。
That is to add a special fluorescence - based dna internal standard in the telomerase elongated ts primers , then do pcr amplification after a step of refinement ( hydroxybenzene / chloroform extracting , and deposited by ethanol ) . sequencing analysis of pcr product was done on 310 gene scan analysis ? . 1 . 2 dna sequencer to determine telomerase activity . notably , this method eliminated the restraining factors of taq dna polymerase , making it possible to erase the sample differences met in pcr and eradicate the annoying phenomena of pseudo negative results
在kim等开发的端粒重复扩增分析法（ trap ）的基础上进行改进，即通过对端粒酶延伸ts寡核昔酸反应产物的精制，消除了pcr扩增中抑制taq酶活性的因素，从而减少了样品之间pcr扩增上的差异和假阴性现象的发生，提高了判断样品端粒酶阴、阳性的准确率和定量的准确性。