遗传性状稳定 meaning in Chinese
stabilization characteristics of genetics
stabilizationcharacteristicsofgenetics
Examples
- In this study , the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome , which have the lacz reporter gene , earlier / latter promoters lp2ep2 of fpv , promoters p7 . 5 and p7 . 1 of vaccinia virus , replication unnecessary region of fpv - 017 . following 6 cycles screenings , clonings , purification of blue plaques , detection of pcr and dot - elisa , which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully . this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine , as well as provided substance preparatory for prevention the high mortality gpv
本研究采用脂质体转染方法,将含有完整gpvh1分离株vp3基因、报告基因lacz 、禽痘病毒早晚期启动子lp2ep2 、痘苗病毒启动子p7 . 5 、 p11和fpv - 017复制非必须区的转移载体质粒psy681vp3lacz与fpv - 017共转染鸡胚成纤维细胞,经6轮蚀斑克隆、筛选、表达, pcr鉴定和dot - elisa检测,证明该重组病毒已构建成功,并获得了遗传性状稳定的鹅细小病毒vp3基因的重组禽痘病毒。 - In nature conditions , without the injection of the extraneous hormone , the females and males of f _ 9 - f _ 12 could mate each other to produce new next generation of tetraploids . with the stable genetic tetraploidy and the fertility in the nature environments , this allotetraploid population possessed the key factors to form a new species with 200 chromosomes
F _ 9 ? f _ ( 12 )染色体数目的稳定性和正常的性腺结构以及自然条件下的生殖传代行为,说明该异源四倍体鲫鲤已成为一个染色体数目为4n = 200 、遗传性状稳定的新型四倍体鱼群体,具备形成一个新的种群所需的关键因素。 - The expression of ha in vero cells infected with rprv - ha was detected by western - blot . the results indicated that ha protein could be consistently detected from a serial passages of vero cells infected with rprv - ha . the recombinant virus can be further developed as a live vectored vaccine against pseudorabies and swine influenza
结果表明所获得的重组病毒( rprvha )遗传性状稳定,在培养细胞中能稳定的表达与sivha具有相似生物学活性的外源蛋白,为进一步制备抗猪流感的重组伪狂犬病毒活载体疫苗奠定了基础。