连续切片 meaning in Chinese
sect serial sections
series section
Examples
- Serial cross sections of a male body
男性连续切片标本 - Fixed with gluteraldehyde , stained with ph 7 . 4 phosphate buffered x - gal dye solution , observed by reverse microscope , the results are as follow : the percent of larvae expressing lacz in midgut showed that fb - 28 improved the sensitivity of larvae to virus
病毒感染后的8h ， 12h ， 16h ， 20h提取中肠组织，按常规石蜡包埋切片进行洗涤、透明、浸蜡、包埋、切片、粘片等操作程序，制成连续切片，在倒置显微镜下逐一进行观察计数。 - The early embryo were made into a series of continuous section slides by tissue cutting . the sections were stained by hematoxylin and eosin ( h & e ) staining and then the development of internal organs such as heart in early embryos was observed by microscope . we found that there is certain relationship between external and internal malformation
同时我们收集人类药物流产的早期胚胎，观察发现胚胎畸形占17 . 86 % ，早期致死占32 . 54 % ;采用组织切片技术将胚胎制成一系列石蜡连续切片，染色后显微观察畸形和正常的早期胚胎内部心脏等器官的发育情况，发现胚胎外部畸形与体内畸形存在一定关联，对此我们将做进一步的研究。 - Material and methods normal rats of male sd were divided into young , adult , and aging groups . preparation of samples for light microscopy : animals were anesthetized by peritoneal injection of 6 % chloral hydrate ( 0 . 5ml / 100g body weight ) . perfusion and fixation of animals were carried out by a common procedure : 37 normal saline 50 - 100 ml and then 4 % paraformaldehyde pbs 100 - 400ml were perfused through the left ventricle of the heart , the whole procedure was lasted for about somin . the entire brain was dissected out and dipped in the fixative solution for 12h at 4 . brain pieces targeted were choosen and then passed the graded alcohols for dehydration , dipping into paraffin for embeding , and reshaping the pieces
2 ）磷酸缓冲液100400m ， 30分钟灌注完毕，取出整脑，在上述固定剂oc ）内后固定12小时。切取观察部位脑块，然后，进行梯度酒精脱水，浸蜡，包埋，修块，石蜡连续切片（德国leica石蜡切片机人切片厚度still ， zlllll ，蛋自甘油载片捞片， 60c烤箱过夜，二甲苯脱蜡，梯度酒精置换，浸水， h六染色，梯度酒精脱水，二甲苯透明，中性树脂封片。室温风干后，显微镜观片， olympus万能显微镜照相。 - The lung tissue for immunohistochemitry and laser scanning confocal microscopy were fixed and embedded . the morphological alteration of pulmonary neuroendocrine cells which stain for calcitonin gene - related peptide ( cgrp ) , serotonin ( s - ht ) and luteinizing hormone ( lh ) were studied . the results of these were dealed with computer image analysis and statistical treatment
肺组织取材后经固定、梯度酒精脱水、包埋、连续切片后，应用免疫组织化学方法、透射电镜及激光扫描共聚焦技术观察了降钙素基因相关肽( cgrp ) 、五羟色胺( 5 - ht ) 、黄体生成素( lh )阳性细胞的随龄变化，并对实验结果进行了计算机图像分析和统计学处理。