转染试剂 meaning in Chinese
transfection reagent
Examples
- 2 . construction of chimeric mtb8 . 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8 . 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8 . 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments , respectively
3 .重组质粒在真核细胞中的表达: pm 、 pms 、 pmi和pmsl重组质粒用lipofectaminatmzo0o脂质体转染试剂转染cos一7细胞，进行瞬时表达， 48小时后，用rl ’ - pcr检测目的基因在mrna水平的表达;用westemblotting检测hil一12在蛋白质水平的表达。 - In order to study the function of cycling2 in vitro culturing cell line , we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent , and studied the function of cycling2 expression on the cell proliferation in vitro , further investigated the regulation mechanism of cycling2 . at the same time , we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue . material and method 1 material : piresneo vector was purchased from clonetech , plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640 , , dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit , mouse monoclonal antibody p21wafl ( in use ) , dab staining kit were purchased from maixin company
实验材料与方法1 .实验试剂高糖dmem 、 rpmll640和胎牛血清购自美国g山eo / brl公司; dmewf12 ( 1 : 1 )混合培养液购自美国hyclone公司;胰蛋白酶购自美国si目叮a公司; hepes由美国amersco公司分装;脂质体转染试剂( upofectalnineplusreageni )和以18为美国玩vitrogen公司产品; piresneo载体购自美国cloneteeh公司;质粒提取及纯化试剂盒购自德国qiagen公司; ultresensitive翎s一p免疫组织化学试剂盒;鼠单克隆抗体户3 ( do一7 )蛋白(即用型) ;鼠单克隆抗体p21waf ， (即用型) ; dab染色试剂盒均购自福建迈新公司;鼠单克隆抗体pziwa曰(浓缩型) ;辣根过氧化酶标记羊抗鼠二抗购自北京中山公司; ecl试剂盒购自美国santacruze公司; dcproteinassay试剂盒购自bi 。