贮存液 meaning in Chinese
stock solution
Examples
- The angiostatin baculovirus transfer vector was co - transfected with viral dna into sf9 cells according to the manufacturers protocol . to purify the recombinant virus , we used the plaque assay to screen the pure recombinant plaque and amplify it to generate p - 1 stock
构建重组病毒：用已经构建好的angiostatin杆状病毒转移载体pbluebachiszb和病毒dna共同转染sfg细胞，通过蚀斑实验筛选出纯的重组斑点并扩增产生p二病毒贮存液。 - Once the recombinant virus has been i - dentified , we amplified the p - 1 stock to attain the large scale , high - titer viral stock in order to initiate expression studies . the recombinant angiostatin was produced from spodoptera frugiperda 9 ( sf9 ) insect cells infected by the high titer virus stock we have prepared . the time course for expression of recombinant protein was detected by sds - page and western blot , which can determine the optimal multiplicity of infection ( moi ) and the appropriate time of harvest for the protein
表达重组蛋白angiostatin ：用制备好的带有矗s标记的融合性angiostatin基因的高滴度重组杆状病毒贮存液感染草地贪夜蛾sfg细胞，用sds page电泳和hsternblot对感染不同时间后分泌的重组蛋白做时间表达分析，依此确定最适的感染复数（ moi ）和感染时间，以达到重组蛋白表达水平最适化，而后大规模进行重组蛋白的表达， sds page用来分析重组蛋白， westernblot用来在蛋白表达水平低的情况下检测表达的特异重组蛋白。