血清学鉴定 meaning in Chinese
serologic identification
serological identification
Examples
- The sucking mouse brain were inoculated with mdj - 01 strain to make electron microscopic examination , results showed that the virus was a spheral particle with membran which had a diameter of about 40 nm . by indirect fluorescent antibody test mdj - 01 strain was identified with tbev . a part of region encoding e protein was expanded by rt - pcr and sequenced . the nucleotide sequences of two strain viruses were compared with sequences in genbankjsequence homology analyses revealed mdj - 01 strain and senzhang strain had the highest homology with tbev oshima5 - 10 , respectively , which were 95 % , 94 % . mdj - 01 strain was identified with tbev again
应用间接免疫荧光试验进行血清学鉴定,结果表明mdj - 01株为tbev 。通过rt - pcr技术扩增部分e蛋白序列并测序,在genbank上进行同源性比较,发现mdj - 01株和森张株与tbevoshima5 - 10株的同源性最高,分别为94 、 95 ,从分子生物学水平上进一步证明mdj - 01株病毒为tbev 。在鉴定的基础上,本实验对两株病毒进行了核苷酸全序列测定。