蛇岛蝮蛇 meaning in Chinese
agkistrodon shedaoensis
Examples
- Cloning and expression of the cdna of a thrombin - like enzyme from the venom of gloydius shedaoensis
大连蛇岛蝮蛇类凝血酶基因克隆与表达研究 - Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ) , strain bl21 ( de3 ) , to study their expression in prokaryotic cell . the gene was expressed under t7 promoter with a fusion protein partner of thx . tag and a 6x his . tag at its n - terminal . having been induced by iptg for 4 hours , the recombinant enzyme was examined in the cytoplasm by sds - page analysis
将大连蛇岛蝮蛇和长白山白眉蝮蛇毒类凝血酶基因分别克隆到大肠杆菌表达载体pet - 32 ( a ) +中，在t7启动子下表达出融合蛋白，融合部分为硫氧还蛋白，位于类凝血酶基因上游，并在其n端带6xhistag标签以利于表达产物的分离纯化，经热激转化至宿主菌bl21 ( de3 )中， iptg诱导斗小时后收获菌体。 - Its amino acid sequence exhibits significant homology with those of other thrombin - like snake venom enzymes . based on the homology , the catalytic residues and disulfide bridges of gloshedobin were deduced to be as follows : his43 , asp88 , ser182 and asp176 ; and disulfide bridges , cys7 - 141 , cys 28 - 44 , cys 76 - 234 , cys120 - 188 , cys152 - 167 and cys178 - 213
以同样的方法，得到长白山白眉蝮蛇( gloydiusussuriensis )毒类凝血酶基因，并比较了它与大连蛇岛蝮蛇类凝血酶基因序列的差别，发现两者的同源性高达98 ，只有三个氨基酸不同： gln ~ ( 66 )