蓝白斑筛选 meaning in Chinese
blue-white selection
Examples
- The immunization effects of b95 can be disturbed by hi20 if the immunized ? interval less than 10 days with the normal dose
将此片段与pbluescriptsk载体连接，转化大肠杆菌tgi ，经蓝白斑筛选，鉴定阳性克隆后测序。 - 713bp and 700bp specific fragments were amplified by pcr and ligated into pgem - t easy vector . it was identified by restriction endonuclease digest analysis , pcr and sequencing that this fragment contained the complete open reading frame ( orf ) of the hc and ha gene
扩增产物连接到pgem - teasy载体上，转化入大肠杆菌jm109中进行蓝白斑筛选后，用酶切、 pcr鉴定和测序的方法鉴定出重组阳性质粒( pgem - hc和pgem - ha ) 。 - The 1 . 488kb dna fragment was sequenced and ligated to pbi121 vector and transformed into otsa deficient of e . coli strains ( ff4169 ) . otsa gene is in charge of trehalose - 6 - phosphate synthesis in e . coli . in growth curve experiment , the transformants that carried the 1 . 488kb dna fragment grew well in minimum medium , which contains 0 . 5mol / l nacl , while control strains could n ' t endure it
提取酿酒酵母的总dna ，以此为模板，采用pcr的方法从酿酒酵母中克隆出了1 . 488kb的海藻糖合成酶基因tps1片段，通过xba和sma双酶切，与同样经过xba和sma双酶切的puc118载体质粒连接，转入大肠杆菌dh5中，通过蓝白斑筛选重组子。 - In order to construct the recombinant plasmid pcdna3 . 1 / ts87 , first , the ts87gene fragment was repcred using the redesigned primers to introduce re sites of hindld and bamh i , and kozak sequence . then the rebuilt ts87 fragment was cloned into t - vector and transformed into e . coli dh5 a
通过重新设计引物在ts87两端加上用于构建重组质粒的酶切位点hind和bamh和用于真核表达的kozak序列。将改造后的基因片段克隆入t - vector ，转化大肠杆菌dh5 ，通过蓝白斑筛选获得阳性克隆后测序鉴定。 - The e2 gene was amplified by rt - pcr , then examined the fragment by electrophoresis . after purification and insertion into pucm - t vecter , the recombinant plasmid pbne2pi and pbne2pii were obtained . then they were transfected e . coli jm109 and screened positive clones by blue or white plaques . the recombinant plasmids were extracted and the inserted fragments were identificated by electrophoresis
通过rt - pcr扩增e _ 2基因，电泳测定扩增片段的大小，经纯化后，连接于pucm - t载体，获得重组质粒pbne2p 、 pbne2p ，转化e ? colijm109 ，经蓝白斑筛选，挑取阳性克隆，提取质粒，直接电泳鉴定和酶切鉴定。