菌丝培养 meaning in Chinese
mycelium culture
Examples
- This experiment carries on culture observation mycelium page , the experiment of antagonism , esterase isozyme analysis and morphological characters to 18 different kinds of ganoderma
摘要对18个灵芝菌株进行了菌丝培养观察、拮抗试验和酯酶同工酶聚丙烯酰胺凝胶电泳实验，并对它们作了聚类统计分析。 - First , after investigation of two original strains " biological characteristics , we studied the main influence factors on protoplasts formation and regeneration in s . mycarofaciens and s . erythreus , and determined the best protoplasts formation and regeneration conditions of two original strains . the former shake - cultured in s " medium at 28 ? , 220r . min ~ ( - 1 ) for 24h , lysised by 3mg / ml lysozyme , keeping warm at 32 ? for 50 ~ 60min , regenerated on r _ ( 5 " ) medium , 28 ? for 5 ~ 6d . the latter used two - step culture , then used img / ml lysozyme keeping warm at 37 ? for ih ; the protoplasts were plated on r5 " regeneration medium at 28 ? for 5d
首先在对两亲株的生物学特性进行了鉴定后，考察了影响两亲株原生质体形成和再生的主要因素，确定了生米卡链霉菌和红霉素链霉菌原生质体形成及再生的最佳条件：前者用s培养基，在28 、 220r . min ~ ( - 1 )培养24h后，用3mg ml的溶菌酶在32恒温酶解50 60min ，得到的原生质体在干燥的r5培养基上28倒置培养5 6天，可得到再生率在20左右的再生菌落；后者采用二级菌丝培养，用1mg ml的溶菌酶在37恒温酶解1h左右，得到的原生质体也在干燥的r5培养基上28倒置培养5天，即可得到再生率在20左右的再生菌。