胞浆区 meaning in Chinese
cytoplasmic domain
Examples
- We confirmed one single nucleotide polymorphism ( snp ) site in the exon 7 of cd226 gene with agt - ggt polymorphism of 307 codon . among ten volunteers , 4 cases are serine and 6 cases are glycine at this codon
在编码cd226胞浆区第307位氨基酸的密码子上存在agt ? ggt单核昔酸多态性（ singlenucleotidepolymorphism ， snp ） ；分别编码丝氨酸和甘氨酸。 - Confocal microscopy observation followed immune - fluorescence staining with anti - gp130 showed that gp130 could interact with tle1 at the cytomembrane region . moreover , this interaction inhibited the concentration of tle1 into nucleus
为了进一步证实上述发现，我们表达并纯化了gst - gp130胞浆区融合蛋白和gst - tle1独特性片段融合蛋白，并制备了特异性抗tle1多克隆抗体。 - By yeast two - hybrid assay , aes was found to interact with gp130 intracellular region through its conserved q domain . results from the yeast two - hybrid assay , gluthione s - transferase fusion protein pull - down assay and immuno - co - precipitation assay indicated that the q domain of tle1 is capable of binding gp130 intracellular domain , and the intracellular membrane proximal region of gp130 containing conserved boxl and box2 motifs seemed essential for this interaction . to investigate the consequence of this interaction , tle1 - gfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with gp130 expression vector
在通过酵母双杂交分析确定aes通过q结构域与sp130分子胞浆区结合的基础上，为确定tle1分子是否也能通过保守的q结构域与gp130分子胞浆区结合，我们通过pcr扩增编码gp130胞浆区与tleq分子不同结构域的cdna ，构建了含有这些不同结构域的酵母双杂交载体，通过酵母双杂交分析证实： tle1分子通过其氨基端的q结构域与gp130分子胞浆区近膜段结合。