缺失法 meaning in Chinese
deletion method
Examples
- The result of blastx shows that one orf is extracellular serine protease precursor gene ; 3 orfs function as regulatory genes ; 5 orfs are involved in secretion pathway ; 2 orfs are related to production of lps ; and the annotation functions of the other 3 orfs are not clearly related to extracellular protease prouduction . marker exchange method was used to study the relationship between the production of protease and the 3 orfs . a deletion mutant of xcc _ 4463 was constructed successfully
Orf注释表明，共中一个基因为胞外蛋白酶结构基因， 3个基因与合成调控有关， 5个与转运分泌有关， 2个与lps合成有关，其余3个orf的注释功能与胞外蛋白酶的关系未见报道，为研究这3个orf与胞外蛋白酶产生的关系，采用同源双交换orf缺失法进行了进一步验证，成功地构建了xcc _ 4463缺失突变体，所得缺失突变体经检测胞外蛋白酶减少，在寄主上致病性降低。