红霉素链霉菌 meaning in Chinese
streptomyces erythraeus
Examples
- First , after investigation of two original strains " biological characteristics , we studied the main influence factors on protoplasts formation and regeneration in s . mycarofaciens and s . erythreus , and determined the best protoplasts formation and regeneration conditions of two original strains . the former shake - cultured in s " medium at 28 ? , 220r . min ~ ( - 1 ) for 24h , lysised by 3mg / ml lysozyme , keeping warm at 32 ? for 50 ~ 60min , regenerated on r _ ( 5 " ) medium , 28 ? for 5 ~ 6d . the latter used two - step culture , then used img / ml lysozyme keeping warm at 37 ? for ih ; the protoplasts were plated on r5 " regeneration medium at 28 ? for 5d
首先在对两亲株的生物学特性进行了鉴定后，考察了影响两亲株原生质体形成和再生的主要因素，确定了生米卡链霉菌和红霉素链霉菌原生质体形成及再生的最佳条件：前者用s培养基，在28 、 220r . min ~ ( - 1 )培养24h后，用3mg ml的溶菌酶在32恒温酶解50 60min ，得到的原生质体在干燥的r5培养基上28倒置培养5 6天，可得到再生率在20左右的再生菌落；后者采用二级菌丝培养，用1mg ml的溶菌酶在37恒温酶解1h左右，得到的原生质体也在干燥的r5培养基上28倒置培养5天，即可得到再生率在20左右的再生菌。 - But in s . mycarofaciens , there is no distinctive propionate kinase , so that the utilization rate of propionic acid is lower than that of acetic acid . for this reason , it is hoped that the higher producers would be obtained by protoplast interspecies fusion between s . mycarofaciens and s . erythreus , which would transfer propionate kinase of s . erythreus to s . mycarofaciens . therefore , the fusion cells could use propionic acid as precursor in synthesis of mdma _ ( 1 ) , which would reduce the production costs
根据文献报道，红霉素链霉菌中的丙酸激酶对丙酸的利用率是对乙酸的利用率的13倍；而在生米卡链霉菌中，无特异的丙酸激酶，菌体对丙酸的利用低于对乙酸的利用，因此希望利用原生质体种间融合的方法将红霉素链霉菌的丙酸激酶基因转移到生米卡链霉菌中，从而使融合子能够利用丙酸盐作为合成mdma _ 1的前体，提高mdma _ 1的产量，降低生产成本。 - 464 and erythromycin producer s . erythreus as original strains , studies were carried out for researching higher producers and enhancing content of midecamycin ( mdm ) ai , which could use propionate as precursor to reduce production costs . according to the literature , the utilization rate of propionic acid is thirteen times to that of acetic acid by propionate kinase in s . erythreus
本论文主要研究了生米卡链霉菌与红霉素链霉菌原生质体种间融合育种，以提高麦迪霉素a _ 1 ( mdma _ 1 )的组分，增加其对丙酸盐的利用度，降低生产成本。