糖基化蛋白 meaning in Chinese
glycosylated protein
Examples
- Mammalian cells are among the best systems for biopharmaceutical production due to their excellence in post - translational modifications . the products they produced are much more similar to their natural forms than those produced by prokaryotic , yeast or insect cells
哺乳动物细胞表达系统具有准确的转录后修饰功能，表达的糖基化蛋白药物在分子结构、理化性质和生物学功能方面最接近天然蛋白分子，是目前重组糖蛋白药物生产的首选体系。 - The recombinants were constructed by transforming ppic9 a - xynb into p . pastoris gs115 . the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p . pastoris . in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity . the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1 . xynbb was gotten by deglycasylation of xynba , whose molecule weight returned to 23kd . we comparised the enzymatic properties of xynba expressed in p . pastoris , xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph , the optimal ph of xynb and xynba were both 5 . 2 , the optimal ph of xynbb was 5 . 0 ; the optimal temperature of xynb and xynba were both 60 c , while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883 . 88iu / mg and 832 . 5hu / mg respectively , which were both lower than 2814 . 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21 . 56 ( g / kg ) and 20 . 87 ( g / kg ) , while the km value of xynbb was 27 . 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg . min and 5329umol / mg . min respectively which were lower than 27623 umol / mg . min of xynb ; additionally all of the three enzymes did not display cellulase activity . they all had well resistance to pepsion and trypsin , and were not sensitive to metal iron , surface active agent and chelating agent . the analysis of different xylans enzymatic hydrolysate revealed : by xynba , that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose , which account for 68 . 43 % and 16 . 50 % respectively , additionally there was 11 . 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose , which account for 81 . 78 % and 11 . 55 % . the result indicated that this xylanase was a kind of 1 , 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
进一步对xynba进行了脱糖基化处理得到xynbb ,其分子量恢复到23kd ，证明xynba是糖基化蛋白。通过对毕赤酵母重组表达的木聚糖酶xynba 、脱糖基化的木聚糖酶xynbb以及橄榄绿链霉菌a1所产原酶xynb之间酶学性质的比较发现：三种酶的最适ph差异不大， xynb和xynba均为5 . 2 ， xynbb为5 . 0 ； xynb和xynba的最适温度均为60 ， xynbb降为50 ：在耐热性上， xynba由于糖基化作用热稳定性明显高于未糖基化的xynb和xynbb ； xynba和xynbb的比活性分别为883 . 88iu mg和832 . 51iu mg ，明显低于原酶的比活2814 . 45iu mg ； xynb和xynba的km值相当，分别为21 . 56 ( g kg )和20 . 87 ( g kg ) ，而xynbb的km值较大为27 . 10 ( g kg ) ； xynba和xynbb的vmax相差不大，分别为4568 mol mg ? min和5329 mol mg ? min ，明显低于xynb的27623 mol mg ? min此外三种酶均无纤维素酶活性，对胃蛋白酶和胰蛋白酶有很好的抗性，且对作用环境中的各种离子、表面活性剂、螯合剂不敏感。通过对不同木聚糖的酶解产物的糖份分析发现：以桦木木聚糖为底物时，酶解产物主要为木三糖和木四糖，含量分别为68 . 43和16 . 50 ，另外还含有11 . 79的木二糖；以玉米芯木聚糖为底物时，酶解产物主要为木二糖和木三糖，含量分别为81 . 78和11 . 55 。