磷酸缓冲液 meaning in Chinese
phosphate buffer
Examples
- Serial paraffin sections were cut with paraffin microtome ( leica ) , thickness of which were 5 y m and 2 n m . mounting the sections on slides with protein and glycerol and then put them in 60 baker overnight
Im ， ph7 2 ）磷酸缓冲液100一400 1 ， 30分钟灌注完毕，取出整脑，在上述固定剂koc ）内后固定12小时，切取观察部位脑块，将切块收入0 - ( 2 ) the adsorption of lysozyme from phosphate - buffered saline ( ph 7 . 4 ) onto bare and cysteine or 1 - octadecanethiol - modified au electrode has been investigated in situ using an electrochemical piezoelectric quartz crystal resonance system ( eqcis )
( 2 )用电化学石英晶体阻抗系统( eqcis )现场监测了ph7 . 4磷酸缓冲液中溶菌酶在金电极和半胱氨酸或正十八硫醇修饰电极上的吸附。 - The mounted sections were dewaxed in xylene and passed in graded alcohols for pcrmutation , dip in distilled watcr . h . e staining was proceeded , then dehydrated in graded alcohols , cleared in xylene , and sealed 8 with neutralized resin
Im 、 ph7 2磷酸缓冲液中过夜后，再用上述磷酸缓冲液冲洗切块三遍，然后梯度酒精脱水，醋酸乙戊酯置换，二氧化碳临界点干燥，真空喷金，日历s七500n观察、照像。 - Without ammonium sulphate fractionation and dialysis , the supernatant of crude extract was directly loaded on deae - sepharose cl 6b column equilibrated by phosphate buffer ( 50mmol / l , ph7 . 8 ) , and the enzyme fraction was not absorbed on the column but impurities were absorbed
粗酶液无需硫酸铵沉淀及透析，即可引入磷酸缓冲液( 50mmol l ， ph7 . 8 )预平衡的deae - sepharosecl6b柱，上柱后用平衡缓冲液洗至基线稳定。 afpga不被吸附而直接流出。 - Material and methods normal rats of male sd were divided into young , adult , and aging groups . preparation of samples for light microscopy : animals were anesthetized by peritoneal injection of 6 % chloral hydrate ( 0 . 5ml / 100g body weight ) . perfusion and fixation of animals were carried out by a common procedure : 37 normal saline 50 - 100 ml and then 4 % paraformaldehyde pbs 100 - 400ml were perfused through the left ventricle of the heart , the whole procedure was lasted for about somin . the entire brain was dissected out and dipped in the fixative solution for 12h at 4 . brain pieces targeted were choosen and then passed the graded alcohols for dehydration , dipping into paraffin for embeding , and reshaping the pieces
2 ）磷酸缓冲液100400m ， 30分钟灌注完毕，取出整脑，在上述固定剂oc ）内后固定12小时。切取观察部位脑块，然后，进行梯度酒精脱水，浸蜡，包埋，修块，石蜡连续切片（德国leica石蜡切片机人切片厚度still ， zlllll ，蛋自甘油载片捞片， 60c烤箱过夜，二甲苯脱蜡，梯度酒精置换，浸水， h六染色，梯度酒精脱水，二甲苯透明，中性树脂封片。室温风干后，显微镜观片， olympus万能显微镜照相。