碱性磷酸酶染色 meaning in Chinese
alkaline phosphatase staining
Examples
- Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml , es - d3 cells being used in our experiments formed normal clones , expressed akp and kept their normal karyotype over many passages . the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers
结果显示： eso3细胞在小鼠胚胎成纤维细胞上和或含白血病抑制因于亿f ）的es细胞培养液中形成典型的胚胎干细胞克隆，碱性磷酸酶染色结果为强阳性，具有正常二倍体核型以及具有在体内外分化为三个胚层来源的组织细胞的潜能，而且具有形成种系嵌合动物的能力。 - Escs can be induced into neuron - like cells by sequential neural induction the undifferentiated escs grew in colonies , with clear bound . alkaline phosphatase staining showed dark brown positive particles were distributed in every esc colony . after the sequential neural induction , the cells converted into neuron - like cells , with homogeneous forms , which had round bright cell bodies and thin long bipolar or multipolar processes
5诱导分化前后细胞端粒酶活性检测( trap )结果1 .使用序贯诱导法可将escs大比例诱导分化为神经元样细胞未分化的escs聚集成团状生长，细胞排列紧密，集落边界清楚，碱性磷酸酶染色呈强阳性，胞浆布满棕黑色颗粒。