硝酸纤维素膜 meaning in Chinese
nitrocellulose membrane
Examples
- We did the same steps for three times , so we could get the extraction using the steps mentioned . laemmli sample buffer was added to the extracted protein , which were then boiled for 5 min . the protein samples were separated by 12 % sds - page and transferred to nitrocellulose membrane
样品蛋白经12 % sds一聚丙烯酞胺凝胶电泳分离后，转印到硝酸纤维素膜上，与第一抗体4孵育过夜，经tbs洗涤后，再与第二抗体室温孵育2小时， ttbs充分冲洗后，显色观察。 - Dab served as chromagen . western blot thirty micrograms of protein extracted from untreated and bfgf , atra - induced mmscs cultures were separated on a 8 % gradient acrylamide gel and eletrophoretically transferred to a nitrocellulose membrane . the blot was probed for nse expression
4westernblot检测诱导后细胞的nse表达情况从未经处理和经过bfgf ， atra诱导的细胞中提取30爬蛋白在8的sds一聚丙烯酸胺凝胶上电泳并转移到硝酸纤维素膜上， 4 5脱脂奶粉封闭过夜。 - The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2 , ha order to prepare ndrg2 antibody , the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector . after induced by iptg , the fusion proteins were expressed in e . coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization , rabbits produced high titer antiserum against ndrg2 . then antisemm was absorbed using ndrg2 antigen immobilized on nc filters , the purified product of antiserum shows high special to ndrg2 protein , and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
为制备高效价的ndrgz抗体，分别构建了prset a雌、 pgex4t d唾仓和pgex4tl七三种原核重组表达质粒，并在大肠杆菌中诱导表达出相应的融合蛋白；用全长gstjqdrgz蛋白免疫兔，然后用gst ndrgz人和gstjqdrgze片段加强免疫，经免疫得到了较高效价的兔抗人ndrz多克隆抗血清，利用固定于硝酸纤维素膜上的ndrgz抗原亲和吸附纯化抗血清，提高了ndrgz抗体的特异性；并对包涵体形式表达的6his ndrgz进行初步的分离纯化。 - Immunized the balb / c mice with mpt64 protein that was transferred to membrane beforehand . the method of subcutaneous embedding was used in the first two vaccinations ; reinforcement vaccination was taken with the sds - page gel supernatant . after immnization , expression of mpt64 in vivo was assessed indirectly by the production of anti - mpt64 antibodies by hlisa , and the average titre of anti - mpt64 antibodies is 1 : 100
采用皮下包埋的方法，用预先转移到硝酸纤维素膜上的表达蛋白免疫小鼠两次，然后再用sds pa e凝胶表达带匀浆上清加强免疫一次，同时设8 g免疫叽性和牛理盐水阴性对照组。 - The diaphragm had the ability to detect the positive serum when it was diluted to 2 ' 11 and so it has good sensitivity ; stored at 4 for at least 7 months , the sensitivity and specificity of the diaphragm did not change , so it has good stability ; when 10 positive serum was detected 3 times , the result is reproducible , so the diaphragm has good reproducible . serums from experimental inoculated piglets was detected . the results showed that when the titer is l : 16 , the pigs were infected with streptococcus suis ; and when 1 : 64 , the pigs could survive after challege with streptococcus suis . all the results have shown that dot - ppa - elisa was a convenient , rapid , sensitive specific useful method for the detection of antibody
该法以硝酸纤维素膜为固相载体，包被膜载抗原制成的诊断膜片具有良好的特异性：不与仔猪副伤寒、猪巴氏杆菌病、猪大肠杆菌病、猪衣原体病、猪瘟、猪细小病毒病、猪伪狂犬病、猪布氏杆菌、猪丹毒阳性血清反应；膜片具有良好的灵敏性，阳性血清作2 ~ ( - 11 )稀释亦能检出；膜片具有良好的稳定性，在4至少能保存7个月，其灵敏性不变。