瞬时转染 meaning in Chinese
transient transfection
Examples
- The l - scfv genes were inserted into the eukaryotic fusion protein expression vector pegfp - n3 and transfected transiently into cos - 7 cells to express respectively
将所构建的单链抗体基因克隆入绿色荧光蛋白融合表达载体pegfp - n3 ，瞬时转染cos - 7细胞，分析其表达情况。 - The expression of scfvs fusion protein were detectable by fluorescence microscope directly and indirect immunofluorescence and immunohistochemistry analysis after transient expression in cos - 7
瞬时转染cos刁细胞后，通过荧光显微镜观察、间接免疫荧光检测、免疫组化检测证实了scfv融合蛋白的表达。 - The recombinant was identified by dual enzyme digestion and the direction of cd40 / pcdna3 was analysed with t7 primer . after being packed by lipofectaminetm2000 , the recombinant was transfected into b lymphocytes . cd40 expression on membrane , cell proliferation and antibodies concentration were detected with flowcytometry , mtt colorimety assay and el1sa , respectively
以脂质体为介质瞬时转染健康人及sle患者b细胞系，利用流式细胞技术检测膜cd40的表达情况，并利用mtt比色法检测细胞的增殖能力， elisa法检测培养液的ig浓度，以研究b细胞在cd40被抑制以后增殖能力、抗体分泌的改变。 - We fused the gfp into the c - terminal region as well as n - terminal region of emt - 1 protein . the result showed that the localization of the protein encoded by the full length emt - 1 cdna was in endoplastic reticulum ( er ) . extracellular region , transmembrane and intracellular region showed the similar cellular localization
我们用绿色荧光蛋白融合于emt l全长及其不同截断形式的梭基和氨基端，瞬时转染cos 7细胞，通过荧光显微镜观察，发现emt l编码的蛋白呈现内质网定位的特点。