真核生物表达 meaning in Chinese
eukaryotic expression
Examples
- At the same time , vp4 gene was mutated in a certain point by pcr . the plant expression vector : pbi121 was constructed and then transformed to agrobacteriutn tumefaciens eha105 directly . a . tumefaciens eha105 harboring pbi121 / vp4 was used to transform the boechmeria nivea l . guad according to the leaf disc procedure
为便于基因操作，对外壳抗原蛋白vp4基因进行适当的修饰和改造：通过引物设计，利用pcr反应，在基因的起始编码前引入有助于真核生物表达的kozak序列和限制性内切酶位点；使用套叠pcr对vp4基因进行定点突变，以便于将改造后的基因插入pbi121构建植物表达载体，通过直接转化法，把pbi121 vp4转入农杆菌eha105 ，构建了农杆菌工程菌。