电穿孔法 meaning in Chinese
electroporation
Examples
- Tagging cells with quantum dots by electroporation
电穿孔法在量子点标记细胞中的应用 - According to the gene sequence and secondary structure of hcv ns5b , we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et . al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion , the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr
然后根据dsrna设计原则，结合nssb基因的序列特征，借助生物信息学软件设计了针对nssb基因的sirnas ，并交由公司化学合成；电穿孔法转染上述稳定转染的细胞克隆，同时分别以非特异的sirnas转染组和空白转染组为对照， dapi染色后通过荧光显微镜和内标化rtpcr检测，初步证实了化学合成的sirnas可以特异阻断nssb基因的表达。