琼脂糖珠 meaning in Chinese
sepharose 4b
Examples
- Second , a prokaryotic expression construct , obtained from invitrogen transformed into prokaryotic and induced to express vp1 protein . the expressed vp1 fusion protein was purified by affinity chromatography using glutathione - agarose resin and used in elisa and western bolt analysis as the antigen . the elisa and western blot results showed that the anti - fmdv antibody was elicited specifically against vp1 antigen
第二，为了得到抗原蛋白，将vp1的原核表达质粒pgex - 4t - vp1转化入大肠杆菌bl21中，经iptg诱导，裂解细胞后用琼脂糖珠进行纯化，用elisa和westernblot进行检测，结果表明诱导表达出所需大小的融合蛋白。