犬冠状病毒 meaning in Chinese
canine coronavirus
ccv
Examples
- By rt - pcr method . we first selected a pair of primers named 2bp and 4bm which was designed in highly conserved 922 nucleotide region in open reading fram ( orf ) ib from coronavirus . this primer could amplify 11 kinds of coronaviruses . after synthesizing this pair of primers , we amplified the target fragement of 251bp from the panda ' s liver - tissue materials and other different passages of culture of this virus . however , the control cell showed negative result
第一对引物为外层引物，可扩增出1086bp的核酸片段；第二对引物为内层引物，可扩增出515bp的核酸片段。该引物以k378为参考株，含有多个兼并碱基，可扩增出包括k378 、 insavc - 1 、 ccv1 - 71 、 nvsl吉林农业大学硕士学位论文大熊猫犬冠状病毒的分离鉴定及s墓因部分序列的测定和分析等多种犬冠状病毒。