物理图谱 meaning in Chinese
physical map
Examples
- But the results of restriction enzyme reaction are not satisfied . there may be two reasons which lead to these results : one is that the restriction enzymes are not suitable for this plasmid , that is to say there are no appropriate cut sites on the plasmid for these restriction enzymes ; and the other is that the purification of the plasmid is not enough for restriction enzyme reaction , and some impurity affects the last results
为了建立质粒的物理图谱，我们对所提取的质粒进行了限制性酶切消化，摘要但是未能取得满意的结果，原因可能有两个：一，所选择的酶不合适，在质粒上没有合适的酶切位点；二，质粒的纯度不够，影响了酶切反应的结果。 - Tandem repeats play a variety of roles in gene expression , regulation and evolution , and are ideal marker in genetic mapping . further more , the techniqe of dna fingerprinting based on the polymorphism of tandem repeat is now widely used in various fields such as medical jurisprudence , etc . the more eye - catching discovery is , in recent years , that some genetic diseases are related with certain trinucleotide repeats
串联重复在基因表达、调控和遗传等方面起着十分重要的作用,同时它因具有高度多态性,已成为基因组遗传图谱和物理图谱的理想界标,另外以串联重复为基础的“ dna指纹技术”在法医学等领域广泛应用。 - Using the monoclonal antibody to rev and the anti - sera against the rev env gp90 - gst fusion protein . the molecular cloned virus was detected by ifa . we also amplified the gp90 from the cells infected with the molecular cloned virus by polymerase chain reaction . all these results indicated the recombinant plasmid containing the total rev genome cdna is infectious
对snv株前病毒全基因组cdna克隆进行酶切，将酶切产物分别克隆进puc18中，分别将各个亚克隆进行测序，按照酶切位点和已知的部分序列以及rev物理图谱将测得的序列进行拼接，完成了rev全基因组序列。 - The work on physical mapping of the chromosome of s . nanchangensis ns3226 was initiated . nearly a full set of chromosomal asei - bamhi fragments of s . nanchangensis ns3226 were cloned and used as probe to hybridized against its genomic library . thirty four asei linking cosmids were observed from 162 hybridizing cosmids and 20 of them showed no obvious overlapping each other by bamhi digestion , suggesting distinct identifications
此外，还开展了南昌链霉菌ns3226染色体物理图谱构建的前期研究工作：基本克隆到了南昌链霉菌ns3226染色体上全套的ase - bamh片段，以它们为探针从南昌链霉菌ns3226的基因文库中钓到164个阳性克隆，并从中筛选到34个ase linkingcosmids ，用bamh进行初步的酶谱分析，结果表明其中有20个cosmids的bamh酶谱相互间没有明显的重叠性。