活性区域 meaning in Chinese

active regions

The full - length sequence , the 3 ' - deletion fragment , the sequence encoding the mature protein and the sequence encoding the conservative domain , were cloned using synthesized primers . recombinant expression vectors were constructed through directional cloning and then host e . coli were transformed by the vectors
设计特异引物克隆得到毒蛋白基因的4个片段，即基因全长、末端缺失片段、编码成熟肽的片段及编码活性区域的片段。
The former expresses recombinant proteins with a 6xhis tag at n - terminal . the fore mentioned four fragments were all used for expression in this system and the mature protein and the conservative domain were effectively expressed while the expression of the other two was unobvious
前者表达n ?末端加6 his标记的融合蛋白，克隆到的4个基因片段均进行了表达，其中成熟肽和活性区域得到了大量表达，蛋白全长和末端缺失片段表达不明显。