洋桔梗 meaning in Chinese

eustoma grandiflorum
eustoma russellianum
lisianthus

Through the orthogonal experiments , a systematic research on the system of bud regenration from the leaf of eustoma russellianum was carried out
通过正交等实验对洋桔梗品种lisablue叶片再生体系进行了系统的研究。
The suitable concentration of hyg to screen for resistant shoots was 50 mg / l . the kind of antibiotics to inhibit the growth of agrobacterium eha105 was cef , of which concentration was 250 mg / l
以洋桔梗叶盘为外植体，通过根瘤农杆菌菌株eha105介导，用构建于质粒pcambia1301的npr1基因转化洋桔梗。
Four hyg - resistant plants , which were chosen randomly from the plants which grown in the field , were detected through gus and pcr . the results confirmed that the npr1 gene have been induced into the genome of eustoma russellianum
随机选取4株移栽成活的具有hyg抗性的洋桔梗植株进行gus染色和pcr分子检测，均为阳性，初步证明外源npr1基因已整合到洋桔梗基因组中。
The result of study on the factors affecting the transformation frequency showed : the leaf disc was pre - cultured for 2 days , infected for 20 minutes , co - cultivated with agrobacterium eha105 for 2 days , which could improve regeneration efficiency . the highest regeneration efficiency was 13 . 3 % in this experiment
实验结果表明：叶片预培养2d ，农杆菌浸染20min ，与农杆菌共培养2d时，筛选培养基上洋桔梗叶盘不定芽分化频率最高，达13 . 3 。