氨基酸密码 meaning in Chinese
amino acid code
Examples
- All the subjects were genotyped by pcr - rflp ( polymerase chain reaction - restriction fragment length polymorphism ) at polymorphic sac i site inside the exon 7 of the ahsg gene . this polymorphism involves a nucleotide substitution of c to g at the middle nucleotide of the codon at amino acid position 238 resulting in the replacement of threonine ( acc ) with serine ( agc )
所有的样本通过聚合酶链式反应?限制性片段长度多态性方法( pcr - rflp )对ahsg基因的第7个外显子内的sac多态性位点进行基因分型，该多态性位点为238号氨基酸密码子中间的碱基c到g的替换，使苏氨酸( thr ， acc )变为丝氨酸( ser ， agc ) 。 - Objective : construct high - level expression system of echistatin in e . coli methods : obtain amino - acid sequence of echistatin from genebank database . considering the bias of usage of 61 available aminoacid codons in e . coli , design the coding sequence of echistatin , synthesize the dna sequence chemically , get single copy coding gene and repeated two copy coding gene of echistatin . insert the sequence into expression vector pbv220 , and more , we construct fusion expression clone of echistatin with pcr , identify the recombinant vector by dna sequencing
目的构建蛇毒锯鳞蝰素( echistatin )的原核高效表达体系方法由genebank数据库检索蛇毒锯鳞蝰素( echistatin )的氨基酸序列，结合大肠杆菌蛋白质合成体系对氨基酸密码子使用的偏爱性，设计了echistatin编码基因，体外人工合成编码基因dna片段，通过适当的限制性内切酶位点插入表达载体pbv220 ，分别构建了echistatin的单拷贝表达克隆、双拷贝串联表达克隆；进一步通过pcr技术构建echistatin的融合表达基因克隆。 - Is toxic to tenebrio molitor l . . the homological cry3aa gene was modified according to the characters of poplus genes and the other features of eukaryotic gene , and was artificially synthesized . the original and modified homological c / y3aa gene were cloned into binary vector pb121 and pbin438 respectively , and transplanted into 84k clone ( p . tomentos x p . glandulossa ) to breed new anti - insects tree species
通过对氨基酸密码子的偏好、使用频率、多聚腺苷酸信号序列( ppss ) 、 mrna不稳定序列( dst )以及kozak等结构的优化改造，人工设计、合成了预期能在杨树中高效表达的毒蛋白基因modifiedcry3aa同源基因； ( 4 )利用强启动子表达载体pet30 - b完成了cry3aa同源基因在大肠杆菌中的表达。