毕赤氏酵母 meaning in Chinese
pichia pastoris
Examples
- Biological assay proved that the expressed product could stimulate the proliferation of cd34 + hematopoietic cells . conclusion : flt - 3 ligand extracellular domain was successfully expressed
体外活性实验表明，毕赤氏酵母表达的重组fl蛋白可以有效地刺激造血干细胞cd34 “的增殖。 - Sds - page showed efficient expression and secretion of the rhfl . the highest yield ( 108 mg / l ) was obtained when expression was induced with 0 . 5 % methanol for 96 hours
Western杂交实验出现了可被抗体特异性识别的杂交带，证实此条带即为重组毕赤氏酵母km7lppicgkil分泌表达的重组fl蛋白。 - The study lays a foundation for further application of the expressed product in expansion of hematopoietic stem cells and tumor immunotherapy . last , recobinant human fl can be used to generate large numbers of antigen - presenting dendritic cells from cd34 + peripheral blood progenitor cells that might be ideally used for immunotherapeutic approaches
综上所述，本实验根据毕赤氏酵母偏爱密码子构建了高效分泌表达fl的工程菌株，其分泌表达的重组fl蛋白具有与天然蛋白相似的生物学功能，为获取大量重组fl蛋白，进一步研究fl的生物功能奠定了工作基础。 - Methods : an artificial gene for fl extracellular domain cdna was synthesized by using favored genetic codons of pichia pastroris . by inserting human fl extracellular domain cdna coding 156 amino acid resi dues into pichia pastoris expression vector ppic9k containing aox1 promoter and the sequences of alpha secreting signal peptides , a recombinant expression plasmid ppic9k - fl was constructed , and integrated into the alcohol oxidase region of the host genome
为了提高外源基因的表达量，我们根据毕赤氏酵母偏爱密码子人工合成了编码fl胞外区156个氨基酸的cdna序列，目的序列被定向克隆到酵母分泌型表达载体ppic9k质粒上，构建ppic9k - fl表达质粒。