植酸钙 meaning in Chinese
calcium phytate
Examples
- The 102 strains which can produce hydrolyzed circles were obtained using alternative medium containing phytate - calcium . after being isolated and purified , these strains were inoculated into fermented medium , shaking in 28 c at 220r / min for 5 days , then their enzymatic activities were determined by ammonium molybdate - phosphate colorimertry under the condition of 37 cand ph2 . 5 . the result showed there were 24 strains with higher enzymatic activities among the 102 strains , after the rescreening , 7 strains were gained with enzymatic activities beyond 15u / ml and stable ability of producing acidic phytase , of which , enzymatic activity of the strain 14 was the highest , reaching 53 . 86u / ml , and it was preliminarilly identified as aspergillus . niger , then numbered as aspergillus . niger 14
用植酸钙选择性平板培养基从土样中筛选出了102株能产透明圈的菌株，经分离纯化后，接入液体发酵培养基， 28 、 220r min发酵5天，在37 、 ph2 . 5条件下用钒钼酸铵法测定其所产植酸酶的活力，结果显示，酶活较高的有24株，经再次摇瓶复筛后，酶活大于15u ml且产酶性能稳定的共有7株，其中以14 ~ #菌株的酶活最高，可达53 . 86u ml ，经初步鉴定为黑曲霉，编号为aspergillus . niger14 ~ # 。 - In total 102 strains of acidic phytase producing strains were selected from soil by selective plate containing calcium phytate . among them 32 strains with relatively large clear circle were purified and re - selected by shaking - culturing . after fermented for 5days at 28 c and shaking at 220r / min , the activity of phytase was determined by nh4vo4 - ( nh4 ) 6mo7o24 method at 37 c and ph2 . 5 or ph5 . 5
主要结果如下： 1植酸酶高产菌株的筛选利用植酸钙选择性子板培养基从土样中筛选出102株酸性植酸酶产生菌，从中挑选出透明圈较大的菌株32株，经分离纯化后分别进行摇瓶复筛， 28 、 220r / min发酵5天后，在37 、 ph2 . 5或ph5 . 5条件下用钒钼酸铵法检测其酶活，结果发现有3株菌产酶活性较高且产酶性能较为稳定。