梯度离心 meaning in Chinese
gradient cen trifugation
gradient centrifugation
gradient-centrifugation
Examples
- Purification : density gradient centrifugation ( sucrose & cscl )
分离:密度梯度离心法(蔗糖&氯化铯) - Density gradient centrifugation
密度梯度离心 - Methods : gradient centrifugation and differential adherence were employed to isolate type pneumocytes from the lungs in the rats , proliferative activities were compared in the cultued cells from the young , adult and aged rats
方法：应用梯度离心和贴壁培养分离大鼠肺泡型细胞，比较幼年、中年和老年大鼠培养肺泡型细胞的增殖能力。 - The fetal liver stem cells were isolated by collagenase digestion , gravity sedimentation and density gradient centrifugation , identified by immunocytochemistry and evaluated by flow cytometry for their proliferation condition
采用胶原酶消化、重力沉降及密度梯度离心方法分离人胎肝干细胞，通过免疫细胞化学方法对其进行初步鉴定，以及应用流式细胞仪等对其生长状况进行评估。 - In the first trial , combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis , and then a modified discontinuous percoll gradient centrifugation method ( 15 % , 22 % , 30 % , 40 % , 50 % , 60 % ) was introduced to isolate spermatids from the cellular suspensions . the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis , and the viability of spermatogenic cells was assessed by using eosin y exclusion test
在第一部分试验中，首先利用连续3次组合酶消化成年小鼠睾丸制备睾丸细胞悬液，然后经6层非连续percoll梯度离心法( 15 、 22 、 30 、 40 、 50和60 )分离，通过形态学和流式细胞术鉴定南京医科大学硕士学位论文各个percoll组分中精子细胞的含量，并以伊红y排斥试验测定细胞的存活率。